{"title":"Effect of Crushed Silver Cyprinid Rastrineobola Argentae Larval Diet on Pupae Eclosion and Mating Success of Male Anopheles arabiensis Mosquitoes","authors":"J. Yugi, O. H. Ochanda, W. Mukabana","doi":"10.5376/JMR.2016.06.0012","DOIUrl":null,"url":null,"abstract":"This study was undertaken to evaluate the effect of larval diet Rastrineobola argentae on pupae eclosion and mating success of adult Anopheles arabiensis Patton (Diptera: Culicidae). Rastrineobola argentae as a test diet and Tetramin ® Baby as a comparative diet were fed to An. arabiensis larvae to generate adults raised exclusively on either of the two diets. Early pupae raised on the diets were placed in containers measuring 6 cm × 5.7 cm × 3.5 cm and assessed for pupae eclosion success. Emerged adults were sexed, transferred to holding cages and provided with 10% sugar solution. Ten three day old virgin male and female raised either on same or different diet types were paired in similar containers as pupae, given 10% sugar solution and left to mate. A wing was removed from experimental mosquitoes and measured to determine size. Spermathecae from every female was removed and examined for sperms. Diet type did not influence pupae eclosion success. Highest proportion of emerged (56%) and competitive (78%) males were associated with crushed silver cyprinid fish food. Female mosquito size influenced mating success (p=0.008) for Tetramin ® Baby fish food raised mosquitoes and not crushed silver cyprinid fish (p=0.068) raised mosquitoes. Most females inseminated were raised from the same diet as the males. Diet type influenced mating success and size. High proportions of larger females were inseminated compared to smaller ones.","PeriodicalId":92079,"journal":{"name":"Journal of mosquito research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of mosquito research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5376/JMR.2016.06.0012","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
This study was undertaken to evaluate the effect of larval diet Rastrineobola argentae on pupae eclosion and mating success of adult Anopheles arabiensis Patton (Diptera: Culicidae). Rastrineobola argentae as a test diet and Tetramin ® Baby as a comparative diet were fed to An. arabiensis larvae to generate adults raised exclusively on either of the two diets. Early pupae raised on the diets were placed in containers measuring 6 cm × 5.7 cm × 3.5 cm and assessed for pupae eclosion success. Emerged adults were sexed, transferred to holding cages and provided with 10% sugar solution. Ten three day old virgin male and female raised either on same or different diet types were paired in similar containers as pupae, given 10% sugar solution and left to mate. A wing was removed from experimental mosquitoes and measured to determine size. Spermathecae from every female was removed and examined for sperms. Diet type did not influence pupae eclosion success. Highest proportion of emerged (56%) and competitive (78%) males were associated with crushed silver cyprinid fish food. Female mosquito size influenced mating success (p=0.008) for Tetramin ® Baby fish food raised mosquitoes and not crushed silver cyprinid fish (p=0.068) raised mosquitoes. Most females inseminated were raised from the same diet as the males. Diet type influenced mating success and size. High proportions of larger females were inseminated compared to smaller ones.
本试验研究了阿氏按蚊幼虫摄食对阿氏按蚊成虫蛹羽化及交配成功率的影响。饲喂试验饲粮为阿根廷拉氏蝽,对照饲粮为Tetramin®Baby。仅在两种饲料中任一种饲养的Arabiensis幼虫产生成虫。将饲粮饲养的早期蛹置于6 cm × 5.7 cm × 3.5 cm的容器中,评估蛹的羽化成功率。出茧成虫进行性别鉴定,转移到笼中,并提供10%的糖溶液。在相同或不同的饮食类型下饲养的10只3天大的雄性和雌性处女在与蛹相似的容器中配对,给予10%的糖溶液,然后离开交配。从实验蚊子身上取下一只翅膀并测量其大小。从每只雌性的精子中取出精子并检查精子。饮食类型不影响蛹羽化成功。出现雄鱼的最高比例(56%)和竞争雄鱼的最高比例(78%)与碾碎的银鲤鱼食有关。雌蚊的大小影响了Tetramin®幼鱼饲料饲养的蚊子的交配成功率(p=0.008),而碾碎的银鲤鱼饲养的蚊子没有影响(p=0.068)。大多数受精后的雌性与雄性的饮食习惯相同。饮食类型影响交配成功率和大小。与较小的雌虫相比,较大的雌虫受精率较高。