Larvicidal, Pupicidal and Smoke Toxic Activity of Alangium salvifolium Leaf Extracts against Culex vishnui Group Mosquitoes

Papiya Ghosh, R. Mondal, K. M. Haldar, G. Chandra
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Abstract

Different vector mosquito species and the diseases spread by them are well studied. Several methodologies have been developed to control those vectors as means to get rid of those diseases with least hazardous effect on environment. The aim of the present study is to evaluate the potentiality of leaf extract of the plant Alangium salvifolium as larvicide, pupicide as well as smoke toxic agent against Culex vishnui group mosquitoes. Various concentrations of crude and Chloroform: Methanol (v/v 1:1) extracts of leaves of A. salvifolium were prepared and applied against each of four successive instars larvae and pupae. In another study the smoke toxicity effect was studied after preparation of the mosquito coils from air dried leaf of the plant. First instar larvae showed 100% mortality at 0.5 mL crude concentration in 24 h. followed by second instar larvae (86.67%) and lastly fourth instars larvae (56.67%). 13.33% death rate was observed for the pupae with same concentration of crude extract. The LC 50 and LC 90 values of the solvent extract were 48.89 and 71.78 ppm respectively. The plant based mosquito coil exhibited 32% mortality against adult mosquitoes within 3 hr. No negative impact was observed on non-target organisms.
丹参叶提取物对vishnui库蚊群的杀幼虫、杀蛹及烟毒活性研究
对不同媒介蚊种及其传播的疾病进行了深入的研究。已经发展了几种方法来控制这些病媒,作为消除对环境危害最小的疾病的手段。本研究的目的是评价桔黄叶提取物作为杀幼虫剂、杀蚊剂和烟毒剂对维氏库蚊群的杀灭潜力。制备了不同浓度的丹参叶粗提取物和氯仿:甲醇(v/v 1:1)提取物,分别施用于连续4个龄期、幼虫和蛹。在另一项研究中,研究了用该植物的风干叶片制备蚊香后的烟毒效应。0.5 mL粗浓度处理24 h, 1龄幼虫死亡率为100%,其次是2龄幼虫(86.67%),最后是4龄幼虫(56.67%)。相同粗提物浓度的蛹死亡率为13.33%。溶剂提取物的lc50和lc90值分别为48.89和71.78 ppm。植物蚊香对成蚊3小时内的死亡率为32%。未观察到对非靶生物的负面影响。
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