Çinko Düzeylerinin Ölçümünde Matriks Seçimi

FORBES Pub Date : 2020-01-01 DOI:10.5222/forbes.2020.09709
Sema Kardeşler, Fatma Demet Arslan, İnanç Karakoyun, Banu Işbilen Başok, Ayfer Çolak
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Abstract

INTRODUCTION: Measurement of blood zinc levels is frequently used in the diagnosis of a few conditions such as growth retardation, immunodeficiency, infertility, neurological disorder, and acrodermatitis enteropathica. The serum matrix is the most preferred one to determine zinc levels due to the ease of analysis with other tests and cost-effectiveness of blood collection tubes used to obtain serum samples. In our study, we aimed to compare serum and plasma zinc test results for accurate and reliable zinc measurement in plain tubes with clot activator and gel and in heparin-free tubes, which specifically produced for trace element analysis. METHODS: Twenty-seven randomly selected patients were included in the study. Blood samples drawn simultaneously from patients were pipetted into tubes with clot activator and gel (SST) (SST II Advance, Vacutainer, Becton Dickinson and Company, USA) as well as into trace element tubes with sodium heparin (NH) (NH Trace Elements Sodium Heparin, Vacuette, Greiner Bio-One GmbH, Austria). After the centrifugation process, zinc levels in serum and plasma samples were analyzed by colorimetric method using an autoanalyzer. Bias between serum and plasma zinc levels was evaluated according to the allowable bias criterion based on biological variation and regression analysis performed. RESULTS: The mean and standard deviation of serum and plasma zinc levels were 49.0±11.5 μg/dL and 46.4±12.0 μg/dL, respectively, and a statistically significant difference was determined (p=0.012) in between. Besides, the bias between serum and plasma was 6.8%, which was above the allowable bias (3.3%) and considered as clinically significant. No systematic or random errors detected. DISCUSSION AND CONCLUSION: When selecting a blood collection tube for trace element analysis, the structural differences between tubes and the matrix effect should be considered carefully.
按汉语水平大小选择矩阵
简介:血锌水平的测量经常用于诊断一些条件,如生长迟缓,免疫缺陷,不孕症,神经系统疾病,肠病性肢端皮炎。血清基质是测定锌水平的最优选方法,因为它易于与其他测试一起分析,而且用于获取血清样本的采血管具有成本效益。在我们的研究中,我们的目的是比较血清和血浆锌的检测结果,以准确可靠地测量锌在普通管与凝块激活剂和凝胶和无肝素管,专门用于微量元素分析。方法:随机选取27例患者进行研究。同时从患者身上提取的血液样本被移入含有凝块激活剂和凝胶(SST)的试管(SST II Advance, Vacutainer, Becton Dickinson and Company, USA)和含有微量元素的肝素钠(NH)的试管(NH微量元素钠肝素,Vacuette, Greiner Bio-One GmbH, Austria)。离心处理后,用自动分析仪比色法分析血清和血浆样品中的锌含量。根据基于生物变异和回归分析的允许偏倚标准评估血清和血浆锌水平之间的偏倚。结果:两组患者血清、血浆锌水平均值为49.0±11.5 μg/dL,标准差为46.4±12.0 μg/dL,差异有统计学意义(p=0.012)。此外,血清和血浆之间的偏差为6.8%,高于允许偏差(3.3%),具有临床意义。没有检测到系统或随机错误。讨论与结论:在选择用于微量元素分析的采血管时,应仔细考虑管的结构差异和基质效应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
FORBES
FORBES BUSINESS, FINANCE-
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