Another Concept of Cancer Interpretation in View of the Interaction between Plasma Radiation and DNA

Abstract

Several studies included our preceding works on different types and doses of plasma sources on both normal and cancerous cells. All previous research tried to discuss and interpret cancer treatment concepts given plasma and cell interaction. Plasma has an effective role and specification character on cancerous cells outcome via many investigations (genetically, immunologically, and biochemically measurements) lead us to numerous paths of different theories which may create a new approach for considerations. This study was designed using a plasma jet powered by a Tesla coil used for generating Cold Atmospheric Pressure Plasma Jet (CAPPJ) from dielectric barrier discharge. The subject samples were categorized into three groups, the first was the cancer cell line. The second was normal blood samples whereas, the third one was non-exposed blood cells cultivated in CAPPJ-exposed cultures. The Cytokinesis Blocked Micronucleus Test (CBMN), a cytome assay, the protein expression of the P53 and Bcl2 genes, the interleukins (IL-1β, IL-6, and IL-10), and tumor necrosis factor (TNF-α) were the variables used in the current investigation. Results indicated that the direct interaction between cells and CAPPJ is more efficient than cells cultivated in CAPPJ-exposed cultures. Cell viability and protein expression levels of Bcl2 and P53 genes in CAPPJ irradiated Breast Cancer Cell lines (BCC) were remarkably valuable. CAPPJ affects cells via not only free radicals and enhancement of several important pathways but may be via direct interaction with DNA.