Evaluation of oxidative stress activity and the levels of homocysteine, vitamin B12, and DNA methylation among women with breast cancer

Q3 Biochemistry, Genetics and Molecular Biology
Rana Rubaye, Rakad Jumaily
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引用次数: 0

Abstract

Objective: This study aims to identify the relationship between oxidative stress, vitamin B12, Homocysteine, and DNA methylation to evaluate their role in the progression of B.C. disease. Methodology: The 5mC global DNA methylation levels in 60 women diagnosed with breast cancer (age range 33–80 yrs.) and 30 age-matched healthy controls were assessed using Methyl Flash™ Methylated DNA Quantification Kit. Patients with B.C. were divided into two groups: group 1 consisted of stage II breast cancer women (Low level), and Group 2 consisted of patients in stage III and IV (High level). Malondialdehyde (MDA), Glutathione peroxidase (GPX), Homocysteine (HCY) and Vitamin B12 levels in the studied subjects were detected by measuring using ELISA. Results: The results showed a significant increase of HCY and MDA in breast cancer patients compared to healthy control, with apparent increases in patients with breast cancer in an advanced stage. They were accompanied by significantly reduced levels of 5mC with a positive correlation between 5mC and different stages of B.C. Also, the results showed that patients in advanced settings and those with a poor prognosis were exposed to low levels of Vit. B12 and GPX (except the patient in stage IV showed increased GPX level). Conclusion: In conclusion, our findings suggest an involvement of Glutathione peroxidase and Homocysteine in the progression of breast cancer, with the potential of utilizing the differences in the levels of global DNA methylation at the different stages as a risk factor for developing the breast cancer.
乳腺癌女性氧化应激活性、同型半胱氨酸、维生素B12和DNA甲基化水平的评估
目的:本研究旨在确定氧化应激、维生素B12、同型半胱氨酸和DNA甲基化之间的关系,以评估它们在bc病进展中的作用。方法:使用methylflash™甲基化DNA定量试剂盒评估60名乳腺癌女性(年龄范围33-80岁)和30名年龄匹配的健康对照者的5mC全球DNA甲基化水平。bc患者被分为两组:组1包括II期乳腺癌妇女(低水平),组2包括III期和IV期患者(高水平)。采用酶联免疫吸附法测定受试者血清丙二醛(MDA)、谷胱甘肽过氧化物酶(GPX)、同型半胱氨酸(HCY)和维生素B12水平。结果:与健康对照组相比,乳腺癌患者的HCY和MDA水平明显升高,晚期乳腺癌患者的HCY和MDA水平明显升高。他们还伴有5mC水平的显著降低,5mC水平与bc的不同阶段呈正相关。此外,研究结果表明,晚期患者和预后较差的患者暴露于低水平的Vit。B12和GPX (IV期患者GPX水平升高除外)。结论:总之,我们的研究结果表明谷胱甘肽过氧化物酶和同型半胱氨酸参与了乳腺癌的发展,并有可能利用不同阶段整体DNA甲基化水平的差异作为乳腺癌发展的危险因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Advanced Biotechnology and Experimental Therapeutics
Journal of Advanced Biotechnology and Experimental Therapeutics Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.90
自引率
0.00%
发文量
41
审稿时长
8 weeks
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