Cloning and Sequence Analysis of the Amylase Gene from the Rice Pest Scirpophaga incertulas Walker and its Inhibitor from Wheat (Variety MP Sehore)

International journal of insect science Pub Date : 2009-01-01 DOI:10.4137/IJIS.S3384

Poonam Sharma, P. Shankar, G. Subramaniam, Amit Kumar, Aanchal Tandon, C. G. Suresh, M. V. Rele, L. S. Kumar

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引用次数: 3

Abstract

Scirpophaga incertulas Walker (Lepidoptera: Pyralideae), commonly known as yellow stem borer, is a predominant monophagous pest of rice, which causes 5% to 30% loss of the rice crop. We report for the first time, the cloning and sequence analysis of the amylase gene of this pest. The cloned gene translates into a protein of 487 amino acids having a predicted molecular weight of 54,955 daltons and a theoretical pI of 5.9. The 3D structure of the amylase is predicted from its amino acid sequence by homology modeling using the structure of the amylase from Tenebrio molitor L (Coleoptera: Tenebrionidae). We also report the purification of a dimeric α-amylase inhibitor from a local variety of wheat MP Sehore that is specific for the amylase of this pest and does not inhibit human salivary amylase or porcine pancreatic amylase. The gene encoding this inhibitor has been cloned and its sequence has been analysed to find a possible explanation for this specificity.

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稻瘟病虫稻卷蝗淀粉酶基因的克隆与序列分析及其抑制物(MP sehor)

黄茎螟(鳞翅目:蚜蝇科)，俗称黄茎螟，是水稻的主要单食害虫，造成水稻作物5%至30%的损失。本文首次报道了该害虫淀粉酶基因的克隆和序列分析。克隆的基因转化成一个由487个氨基酸组成的蛋白质，预计分子量为54955道尔顿，理论pI为5.9。利用拟黄粉甲(鞘翅目:拟黄粉甲科)淀粉酶的氨基酸序列进行同源性建模，预测了该淀粉酶的三维结构。我们还报道了从当地小麦品种MP sehor中纯化二聚体α-淀粉酶抑制剂，该抑制剂对这种害虫的淀粉酶具有特异性，并且不抑制人类唾液淀粉酶或猪胰腺淀粉酶。编码这种抑制剂的基因已被克隆，其序列已被分析，以找到这种特异性的可能解释。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International journal of insect science

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