The efficient method for in vitro micropropagation of Ginkgo biloba L.

Abstract

Background : Ginkgo biloba L. leaf extract is used in medicine due to its therapeutic actions such as regulating cerebral blood flow, lowering oxidative stress, delaying the progress of dementia and diabetes. Objective: At the current study, the efficient micropropagation of G. biloba has been optimized by applying tissue culture method. Methods: Different explants (leaves, stems and lateral buds) were disinfected superficially and were cultured in WPM supplemented with various types and concentrations of plant growth regulators for shooting and subsequently for root induction. Results: The best treatment for shoot induction frequency (100 %), regenerated shoot length (2.47 cm) and number of regenerated leaves per explant (6.5) was achieved by culturing the lateral buds on WPM medium having Kin at 1 mg/L and IAA at 0.5 mg/L. The best root induction medium on the basis of root induction (100 %) and regenerated root lengths (8.5 cm) was WPM medium with IBA at 1 mg/L and AC at 2 g/L. After acclimatization, 60 % of regenerated platelets were survived. Finally, based on HPLC analysis, no significant difference was observed between the amount of quercetin in the leaves of propagated seedlings under in vitro conditions and their mother base. Conclusion: The optimized protocol proposed to be used as an efficient method for commercial micropropagation of ginkgo tree.