In vitro hepatoprotective activity of a polyherbal formulation on HepG2 cell line

Ancient Science of Life Pub Date : 2019-01-01 DOI:10.4103/asl.ASL_57_18

B. Bhatt, A. Dey, Satyajyoti Kanjilal, H. Srikanth, R. Biswas, P. Chakraborty, D. Gandhi, A. Narwaria, C. Katiyar

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Abstract

Background: In Ayurveda, use of multiple herbs in a single formulation is popular and polyherbal Ayurvedic formulations are widely used as growth promoters, gastrointestinal and hepatic regulators, hepatic tonics and so on. Despite the widespread use, there is a lack of scientific evidence on their efficacy and safety. Aim: This study was undertaken to validate the efficacy by evaluating the hepatoprotective activity of one such polyherbal blend (LIVT) on HepG2 cell line. Materials and Methods: Four doses of test formulation (62.5, 125, 250 and 500 μg/ml) were tested on D-galactosamine induced HepG2 cell toxicity model. MTT assay was performed to determine the dose range for the hepatoprotective study. Results: The test formulation exhibited significant (P < 0.05) cytoprotective activity with a maximum protection of 37% at dose 62.5 μg/ml, and the activity was comparable with that of the standard, silymarin. Conclusion: The results of the present study indicate that the polyherbal blend demonstrated a significant hepatoprotective activity and could be used as an active herbal alternative for the treatment of liver ailments.

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复方制剂对HepG2细胞株的体外保肝活性

背景:在阿育吠陀中，多种草药在单一配方中使用很受欢迎，多草药阿育吠陀配方被广泛用于生长促进剂、胃肠和肝脏调节剂、肝脏滋补剂等。尽管被广泛使用，但缺乏关于其有效性和安全性的科学证据。目的:通过评价一种复方中草药合剂(LIVT)对HepG2细胞的保护作用，验证其对肝脏的保护作用。材料与方法:采用62.5、125、250、500 μg/ml 4种剂量的试验制剂对d -半乳糖胺诱导的HepG2细胞毒性模型进行实验。采用MTT试验确定肝保护研究的剂量范围。结果:该制剂在62.5 μg/ml剂量下具有显著的细胞保护活性(P < 0.05)，最大保护率为37%，与标准水飞蓟素的细胞保护活性相当。结论:本研究结果表明，复方中药具有明显的保肝作用，可作为治疗肝脏疾病的有效中药替代品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Ancient Science of Life

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39 weeks