In vitro antioxidant and antimicrobial activities of ethanolic extracts from whole plants of three Impatiens species (balsaminaceae)

F. Delgado-Rodríguez, Olman Hidalgo, Arlene Loría-Gutiérrez, Nien Weng-Huang
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引用次数: 8

Abstract

Context: Plants of Impatiens genus are recognized source of extracts with antioxidant and antimicrobial activity, including I. balsamina(Sanskrit name: Tairini), a plant traditionally used in Asia to treat infections and inflammation. However, there is little information related to activities of whole plant extracts. Aim: To investigate the in vitro antioxidant and antimicrobial activities of the ethanolic extract from whole plant of I. balsamina, I. hawkeri and I. walleriana. Methods: Whole plant material of each species was macerated with ethanol (80% v/v). Phytochemical screening was applied for detection of different metabolites. Total phenolic content (TPC) and total flavonoids content (TFC) were determined using Folin-Ciocalteu and aluminum chloride methods respectively. In vitro antioxidant properties were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, potassium ferricyanide antioxidant reducing power (PFRAP) and oxygen radical absorbance capacity (ORAC) assays. In vitro antimicrobial activity of extracts was evaluated against Staphylococcus aureus(ATCC 29737), Staphylococcus epidermidis (ATCC 12228), Streptococcus pyogenes (ATCC 19615), Streptococcus pneumoniae (clinical isolate), Pseudomonas aeruginosa (ATCC 9027), Escherichia coli(ATCC 10536), Candida albicans (ATCC 10231) and Aspergillus niger (ATCC 16404) using microdilution assay for minimal inhibitory concentration (MIC) assessment. Statistical Analysis Used: Results were expressed as mean ± SD. Data obtained were analyzed statistically using ANOVA (one-way) followed by Tukey's post hoc test. Correlation analyses were performed using Pearson correlation test. Results: I. hawkeri extract showed the highest phenolic content (TPC: 44.04 mg GAE/g; TFC: 55, 02 mg QE/g) and was the most antioxidant extract. I. balsamina extract was the most active against all Gram positive bacteria (MIC: 2.5-10 mg/ml) tested and> over C. albicans (MIC: 10 mg/ml). This extract also showed the widest antimicrobial spectrum. Conclusions: The evaluated Impatiens whole plant extracts are also promising sources of antioxidant and antimicrobial agents.
三种凤仙花(香脂科)全株乙醇提取物的体外抗氧化和抗菌活性
背景:凤仙花属植物是公认的具有抗氧化和抗菌活性的提取物来源,包括凤仙花(梵文名称:Tairini),一种在亚洲传统上用于治疗感染和炎症的植物。然而,有关全植物提取物活性的信息很少。目的:研究香薷、hawkeri和walleriana全株乙醇提取物的体外抗氧化和抗菌活性。方法:采用乙醇(80% v/v)浸泡整株植物。采用植物化学筛选法检测不同代谢产物。采用福林- ciocalteu法和氯化铝法分别测定总酚含量(TPC)和总黄酮含量(TFC)。采用2,2-二苯基-1-苦味酰肼(DPPH)自由基清除能力、铁氰化钾抗氧化还原能力(PFRAP)和氧自由基吸收能力(ORAC)等方法评价其体外抗氧化性能。采用微量稀释法对金黄色葡萄球菌(ATCC 29737)、表皮葡萄球菌(ATCC 12228)、化脓性链球菌(ATCC 19615)、肺炎链球菌(临床分离株)、铜绿假单胞菌(ATCC 9027)、大肠杆菌(ATCC 10536)、白色念珠菌(ATCC 10231)和黑曲霉(ATCC 16404)进行体外抑菌活性评估。统计分析方法:结果以mean±SD表示。所得数据采用单因素方差分析(ANOVA)进行统计学分析,然后进行Tukey事后检验。采用Pearson相关检验进行相关分析。结果:hawkeri提取物中酚类物质含量最高(TPC: 44.04 mg GAE/g;TFC: 55, 02 mg QE/g),是最具抗氧化性的提取物。香脂提取物对革兰氏阳性菌(MIC: 2.5 ~ 10 mg/ml)的抑制作用最强,对白色念珠菌(MIC: 10 mg/ml)的抑制作用最强。该提取物也显示出最广泛的抗菌谱。结论:所评价的凤仙花全植物提取物具有良好的抗氧化和抗菌活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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