In Vitro Goat Fermentation of PUFA-Diet Supplemented with Yeast and Curcuma xanthorrhiza Roxb

Media Peternakan Pub Date : 2014-12-17 DOI:10.5398/medpet.2014.37.3.175

E. Sulistyowati, A. Sudarman, K. G. Wiryawan, T. Toharmat

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引用次数: 7

Abstract

This in vitro experiment was conducted to evaluate the ruminal performances of polyunsaturated fatty acid (PUFA)-diet (containing PUFA with 80% concentrate and 20% King grass) supplemented with yeast and C. xanthorrhiza Roxb. Experimental design was completely randomized block design of 4 x 4 with ruminal liquor derived from 4 slaughtered goats and 4 treatments (PD0-no supplement, PDY- 0.5% yeast, PDC-2% curcuma, and PDM- 0.5% yeast + 2% curcuma). Variables measured were pH, N-NH 3 , total and partial VFA (volatile fatty acid), protozoa population, and CH 4 (methane). Results showed that the lowest (P<0.05) organic (59.63%) and dry matter (58.00%) digestibilities were found in PDM. In in vitro , this diet was also showing quantitatively low in N-NH 3 (8.73 mM) and protozoa population (7.90±4.09 10 3 cfu/mL). On the other hand, it showed numerically high in VFA production (45.27 mM) and pH (6.74), yet low in CH4 (13.43% v/v). Based on these data, PDM was considered the most potential diet to improve nutrient metabolism in rumen of goat, in vitro . Key words: PUFA- diet, yeast, curcuma, in vitro fermentation

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添加酵母和姜黄的pufa日粮的山羊体外发酵研究

本试验旨在评价添加酵母和黄原菌的多不饱和脂肪酸(PUFA)饲粮(PUFA精料80%，王草20%)的瘤胃生产性能。试验设计为4 × 4的完全随机区组设计，采用4只屠宰山羊的瘤胃液和4种处理(pd0 -不添加、PDY- 0.5%酵母、PDM- 2%姜黄、PDM- 0.5%酵母+ 2%姜黄)。测量的变量包括pH、n - nh3、总挥发性脂肪酸(VFA)和部分挥发性脂肪酸(VFA)、原生动物种群和ch4(甲烷)。结果表明:PDM的有机消化率(59.63%)和干物质消化率(58.00%)最低(P<0.05);在体外试验中，n - nh3含量(8.73 mM)和原虫数量(7.90±4.09 10 3 cfu/mL)也较低。另一方面，VFA产量(45.27 mM)和pH值(6.74)较高，CH4含量较低(13.43% v/v)。基于这些数据，PDM被认为是体外最有可能改善山羊瘤胃营养物质代谢的饲粮。关键词:PUFA-日粮，酵母，姜黄，体外发酵

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Media Peternakan

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14 weeks