Evaluation of cold shock-induced cytotoxicity and genotoxicity in the house fly Musca domestica

Q3 Agricultural and Biological Sciences
N. Mishra, R. Tewari
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引用次数: 3

Abstract

Background: Low temperature affects the survival, growth and development of invertebrates, especially insects, based on the severity of cold and the duration of exposure. Although the effects of cold shock or direct chilling were previously analysed in terms of development patterns and defects, morphological changes, cold hardiness, cryopreservation and diapause in insects, very little information is available regarding the effects of cold shock at the chromosomal level. Material and Methods: Late third instar larvae of the house fly Musca domestica were exposed to low temperatures (10, 4, 0 and -5°C) for different durations, in order to assess genotoxicity and cytotoxicity in the present study. The chromosomal aberration assay and micronucleus test were used as genotoxic end points. Cytotoxicity was evaluated by the mitotic index and the extent of tissue damage was observed using the Trypan blue staining method. Results: A significant (P Conclusions: The present work suggests that cold shock induces chromosome aberrations and cytotoxicity and affects the developmental pattern in house fly, M. domestica.
家蝇冷休克诱导的细胞毒性和遗传毒性评价
背景:低温会影响无脊椎动物,尤其是昆虫的生存、生长和发育,这取决于低温的严重程度和暴露时间。虽然以前已经从昆虫的发育模式和缺陷、形态变化、抗寒性、低温保存和滞育等方面分析了冷冲击或直接冷却的影响,但关于冷冲击在染色体水平上的影响的信息很少。材料与方法:将家蝇晚期3龄幼虫置于低温(10、4、0和-5℃)下不同时间,以评估其遗传毒性和细胞毒性。以染色体畸变测定和微核试验作为遗传毒性终点。用有丝分裂指数评价细胞毒性,台盼蓝染色法观察组织损伤程度。结论:冷休克可引起家蝇染色体畸变和细胞毒性,影响家蝇的发育模式。
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来源期刊
Eurasian Journal of Biosciences
Eurasian Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.20
自引率
0.00%
发文量
0
期刊介绍: EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology
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