Caveolin-1 Inhibits Proliferation and Migration of Gastric Cancer Cell via Inactivating BMI-1

Abstract

Objective: To investigate the effect of Caveolin-1on the proliferation and migration ability in gastric carcinoma cells MGC-803 and its mechanism. Methods: Plasmid DNA pcDNA3.1-Cav1 was extracted by extracted kits, and transfected Cav-1 gene sequences were found. The expression levels of Cav-1 protein were detected by Western blot. And the proliferation was analyzed by CCK8 assay. The effect of Cav-1 on migration was detected by wound healing. The expression levels of BMI-1 protein were detected by western blot. Results: 1) Western Blot showed that the expression levels of Cav-1 were higher in MGC-803/Cav-1 than control group, P < 0.05. 2) CCK8 showed, that the proliferation of MGC-803/Cav-1 was inhibited, but P > 0.05. Wound healing showed, that the migration ability of MGC-803/Cav-1 fell off, P < 0.05. 3) Western Blot showed that the expression levels of BMI-1 were lower in MGC-803/Cav-1 than in the control group, P < 0.05. Conclusion: Cave-olin-1 can inhibit the proliferation and migration ability of gastric carcinoma cells and its mechanism may relate to BMI-1.