Treatment Effect of Various Concentration of Plant Extracts on Murine Norovirus

微生物学(英文) Pub Date : 2022-01-01 DOI:10.4236/aim.2022.124018

Uchenna B. Iloghalu, Sara Miller, A. Ewunkem, J. Khatiwada, L. Williams

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引用次数: 1

Abstract

Noroviruses are positive-sense, single-stranded, non-enveloped RNA virus that measures approximately 27 - 35 nm in diameter. It affects humans of all ages and races causing most cases of viral gastroenteritis worldwide. Infection results from ingestion of contaminated food or water as well as causing diarrhea and vomiting in humans. Extracts from plants are known to have antioxidant, anti-inflammatory, and adhesive properties which are associated with barrier functions. The aim of this study was to elucidate whether plaque reduction was due to an effect of methanolic plant extract directly on the virus, whether the extract affects viral replication, and lastly, whether the extract disrupts the cell surface binding with the virus. The plant extracts of interest were the calyces of Hibiscus sabdariffa (HS) and the seeds of Zanthoxylum armatum (ZA). Antiviral activities of these extracts were determined against murine norovirus. The logarithmic viral reduction per pla-que-forming unit was (22 log 10 ) PFU/ml (control), (15 log 10 ) PFU/ml (treated HS), and (12 log 10 ) PFU/ml (treated ZA) with a significant reduction (68% and

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不同浓度植物提取物对小鼠诺如病毒的治疗作用

诺如病毒是正义、单链、无包膜RNA病毒，直径约27 - 35nm。它影响所有年龄和种族的人类，导致世界上大多数病毒性肠胃炎病例。感染起因于摄入受污染的食物或水，以及引起人类腹泻和呕吐。众所周知，植物提取物具有抗氧化、抗炎和与屏障功能相关的粘附特性。本研究的目的是阐明斑块减少是否由于甲醇植物提取物直接作用于病毒，提取物是否影响病毒复制，最后，提取物是否破坏细胞表面与病毒的结合。研究的植物提取物为芙蓉花萼(HS)和花椒种子(ZA)。测定了这些提取物对小鼠诺如病毒的抗病毒活性。每个斑块形成单位的对数病毒减少量为(22 log 10) PFU/ml(对照组)，(15 log 10) PFU/ml(治疗HS)和(12 log 10) PFU/ml(治疗ZA)，显著减少(68%和68%)

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

微生物学(英文)

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661