Virulence Gene Characterization and Serotyping of Major Bacterial Pathogens Isolated from Bovine Respiratory Disease in Ethiopia

Mirtneh Akalu, T. Abayneh, E. Gelaye, Getaw Derese, Behailu Tefera, T. Degefa, Vemulapati Bhadra Murthy
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Abstract

Bovine Respiratory Disease (BRD) causes a severe form of pneumonia in all age of cattle. This study was designed to investigate the distribution of capsular types, serotypes, and virulence-associated genes of the major bacterial pathogens from BRD outbreak samples in Ethiopia. In this study 166 samples were collected from clinically sick (n = 107) and pneumonic lung tissue (n = 59). Laboratory assay confirmed isolation of M. haemolytica 37 (22.29%), P. multocida 25 (15.06%), B. trehalosi 12 (7.23%), and H. somni 15 (9.04%). PCR assay of P. multocida capsular typing revealed 21 (84.0%) cap A (hyaD-hyaC) and 4 (16.0%) cap D (dcbF) strains. M. haemolytica serotypes belonged to A: 1, A: 2, and A: 6 from 26 (70.27%), 4 (10.81%), and 7 (18.92%) isolates, respectively. P. multocida biotyping showed isolation of A: 1, A: 2, and A: 3 from 3 (14.29%), 2 (9.52%),
埃塞俄比亚牛呼吸道疾病主要病原菌的毒力基因鉴定和血清分型
牛呼吸道疾病(BRD)在所有年龄的牛中引起一种严重的肺炎。本研究旨在调查埃塞俄比亚BRD暴发样本中主要细菌病原体的荚膜型、血清型和毒力相关基因的分布。本研究共收集临床病人(107例)和肺炎肺组织(59例)166份样本。实验室鉴定分离出溶血支原体37(22.29%)、多杀假体25(15.06%)、海藻藻支原体12(7.23%)和嗜睡支原体15(9.04%)。多杀假单胞菌荚膜分型结果显示,A帽(hyaD-hyaC) 21株(84.0%),D帽(dcbF) 4株(16.0%)。从26株(70.27%)、4株(10.81%)和7株(18.92%)分离得到溶血分枝杆菌血清型分别为A: 1、A: 2和A: 6。多杀假单胞菌生物分型结果显示,从3株中分离出A: 1、A: 2、A: 3株(14.29%)、2株(9.52%);
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