A Novel Exo-Glucanase Explored from a Meyerozyma sp. Fungal Strain

酶研究进展(英文) Pub Date : 2015-08-25 DOI:10.4236/AER.2015.33006

H. Kuo, J. Zeng, Pinyi Wang, Wen-Chin Chen

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引用次数: 6

Abstract

Isolating cellulase-secreting microbes followed-by screening their cellulolytic activities has been an essential approach to discover novel and potential cellulases for cellulolytic industrial applications. This study was aimed to explore competitive exoglucanases by screening avicelase activities for 92 fungal strains isolated from environmental airborne-fungal-spore samples. Results showed that an isolated fungal strain numbered 58 exhibited the best avicelase activity of 0.209 U/mL when cultured for six days at pH 5.0 - 5.3 and 25℃ - 27℃, and was lately identified as a yeast strain of Meyerozyma sp. (96% ITS fragment similar with Meyerozyma caribbica, HG970748). Based on amino acid sequences revealed from LC/MS/MS, the target exoglucanase was identical to 1,4-beta-D-glucan cellobiohydrolases and was named Mc-CBHI which had optimal avicelase reaction conditions of pH 5 and 70℃ and could remain fairly stable after 4hr incubation at acid conditions (pH 3 - 5) or wide temperature ranges (30℃ - 80℃). Additionally, the Mc-CBHI (~70 kDa and ~3.6% of crude enzyme) had specific FPase and avicelase activities of 0.179 U/mg and 0.126 U/mg, respectively (which were about 40% - 50% activities of a commercial cellulase Accellerase-1000). These results demonstrated that the newly-found Mc-CBHI could become one of potential exoglucanase resources for related cellulolytic industrial applications.

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从Meyerozyma sp.真菌菌株中发现一种新的外链葡聚糖酶

分离分泌纤维素酶的微生物，然后筛选它们的纤维素水解活性，是发现新的和潜在的纤维素酶用于纤维素水解工业应用的重要方法。本研究旨在通过筛选从环境空气传播真菌孢子中分离的92株真菌的乙酰化酶活性来探索竞争性外葡聚糖酶。结果表明，分离得到的58号真菌在pH 5.0 ~ 5.3、25℃~ 27℃条件下培养6 d，酶活性最高，为0.209 U/mL，经鉴定为Meyerozyma sp.酵母菌(96% ITS片段与加勒比Meyerozyma, HG970748相似)。根据LC/MS/MS显示的氨基酸序列，目标外葡聚糖酶与1,4- β - d -葡聚糖纤维素生物水解酶相同，命名为mc - chi，其最佳乙酰化酶反应条件为pH 5和70℃，在酸性条件(pH 3 ~ 5)或较宽温度范围(30℃~ 80℃)下培养4h后保持相当稳定。此外，mc - chi (~70 kDa和~3.6%的粗酶)的特异性FPase和avicase活性分别为0.179 U/mg和0.126 U/mg(约为商品纤维素酶加速酶-1000的40% - 50%)。这些结果表明，新发现的mc - chi可能成为纤维素水解工业应用的潜在外葡聚糖酶资源之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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酶研究进展(英文)

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