Rubber Seed Kernel as Potent Solid Substrate for the Production of Lipase by Pseudomonas aeruginosa Strain BUP2

K. N. Unni, P. Faisal, P. Priji, S. Sajith, S. Sreedevi, E. S. Hareesh, Trikariyoor Asokan Nidheesh Roy, S. Benjamin
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引用次数: 1

Abstract

This study explored the utility of flours of rubber seed, coconut and groundnut kernels, and de-oiled cakes of coconut and groundnut as solid substrate for the production of lipase by Pseudomonas aeruginosa strain BUP2 (MTCC No. 5924), a novel bacterium reported from the rumen of Malabari goat. Various proportions (10%, 20%, 30%, 40% or 50%) of flours or cakes were prepared (w/v) with BUP medium (pH 4, 5, 6, 7 or 8), and incubated at different temperature (25°C, 28°C, 30°C or 32°C) for 24 to 96 h. The samples were assayed for lipase activity at 24 h intervals. The rubber seed flour (20%)-BUP medium supported the maximum lipase production (871 U/gds) at 48h incubation (pH 6, 28°C), followed by ground nut flour (398 U/gds), while ground nut cake supported the least lipase production (244 U/gds). From this, it is evident that the cheaply available rubber seed is an efficient substrate for the production of lipase, irrespective of its known demerit that it contains the limarin, a toxin; in fact, we could not detect limarin in the fermented matter. Thus, the utility of rubber seed for the production of a costly enzyme is reported from a novel rumen bacterium, which would be advantageous for rubber farmers.
橡胶种子仁作为铜绿假单胞菌BUP2产脂酶的有效固体底物
本研究探索了橡胶籽、椰子和花生仁的面粉,以及椰子和花生的去油饼作为固体底物,利用来自马拉巴里山羊瘤胃的新型细菌铜绿假单胞菌BUP2 (MTCC No. 5924)生产脂肪酶。用BUP培养基(pH值为4、5、6、7或8)配制不同比例(10%、20%、30%、40%或50%)的面粉或蛋糕(w/v),在不同温度(25°C、28°C、30°C或32°C)下孵育24至96小时。每隔24小时检测样品的脂肪酶活性。在pH 6, 28°C条件下,橡胶籽粉(20%)-BUP培养基在培养48h时脂肪酶产量最高(871 U/gds),其次是坚果粉(398 U/gds),而坚果饼的脂肪酶产量最低(244 U/gds)。由此可见,廉价的橡胶籽是生产脂肪酶的有效底物,尽管其已知的缺点是含有一种毒素——蓖麻素;事实上,我们无法在发酵物中检测到柠檬素。因此,从一种新的瘤胃细菌中提取橡胶种子用于生产一种昂贵的酶,这对橡胶农民来说是有利的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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