Rabies lyssavirus Isolates from Brazilian Different Reservoirs Species PresentDistinct Pattern of Propagation in N2a Cell

Abstract

Background: Rabies cell culture infection test was developed for the isolation of Rabies lyssavirus and as an alternative for the mouse inoculation test. However, tissue culture for street rabies strains produces low viral titer. Here, we assessed the quantity of brain tissue for successful viral isolation toward increased virus titer in effective way. Methods: Brain tissue isolates from different reservoirs species of Brazil were harvested in different concentration and inoculated in mouse neuroblastoma cells (N2a). These isolates were measured infectious viral titer and cell viability followed by consecutive passages in N2a cells. Results: Inoculum containing were prominent Rabies lyssavirus due to higher viral titer and not significantly dead cell. After consecutive passages in N2a cells Rabies lyssavirus variant maintained by vampire bat had remarkable adaptation to the culture system, while isolates from marmoset presents distinct pattern of propagation in N2a cell when compared with other groups. Conclusion: Based on these results, the isolation followed by viral replication assay may be used in isolates from different reservoirs which enable an effective amplification of the wild type virus strains.