Development of a core collection for Argan tree in Morocco by heuristic search based on SSR markers

IF 0.5 4区医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Ethiopian Journal of Health Development Pub Date : 2017-07-18 DOI:10.4172/2157-7625-C1-030

Derese Hailu, A. Derbie, D. Mekonnen, Yohannes Zenebe, Yesuf Adem, Seble Worku, F. Biadglegne

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引用次数: 16

Abstract

Background: An increased antibiotic resistance of bacterial isolates from wound infections is a major therapeutic challenge. The aim of this study was to identify bacterial isolates associated with wound infection and to determine their current antimicrobial susceptibility profile. Methods: This is a retrospective cross-sectional study in which we analyzed the records of 380 wound swab culture results that have been processed at Bahir Dar Regional Health Research Laboratory Center in the period of 1 January 2013 to 30 December 2015. Swabs from different wound types were collected aseptically and analyzed using standard bacteriological procedures. Antimicrobial susceptibility testing was performed using disc diffusion technique as per the standard protocol. Demographic and bacteriological data were collected using a data extraction sheet. The data were cleaned, entered and analyzed using SPSS version 22. Results: The overall bacterial isolation rate was at 61.6% (234/380). More than half 123 (52.6%) of the isolates were gram positive and 111 (47.4%) were gram negatives. The predominant isolates were S. aureus a t 100 (42.7%) followed by E. coli , 33 (14.1%), P. aeruginosa , 26 (11.1%) and S. pyogenes , at 23 (9.8%). The proportion of multidrug resistant (MDR) bacterial pathogens was at 54.3%. Out of these, 35 (15.1%) of the isolates were resistant to more than five drugs. The highest resistance rate at (85.9%) was documented for ampicillin by gram-negative isolates. Whereas the highest resistance rate among gram positive isolates was against erythromycin (31.1%). The resistance rate of S. aureus for penicillin was at 69.7%. Conclusions: High frequency of mono and multi-drug resistant bacterial pathogens were documented. Thus, an alternative method to the causative agent and antimicrobial susceptibility testing surveillance in areas where there is no culture facility is needed to assist health professionals for the selection of appropriate antibiotics. [ Ethiop. J. Health Dev . 2016;30(3):112-117] Keywords: Wound infection, bacterial isolates, and antimicrobial susceptibility profile

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基于SSR标记的启发式搜索法开发摩洛哥摩洛哥坚果树核心种质

背景:伤口感染中分离的细菌耐药性增加是一个主要的治疗挑战。本研究的目的是鉴定与伤口感染相关的细菌分离株，并确定其当前的抗菌药物敏感性概况。方法:采用回顾性横断面研究方法，对2013年1月1日至2015年12月30日在Bahir Dar地区卫生研究实验室中心处理的380例伤口拭子培养结果进行分析。无菌收集不同伤口类型的拭子，并使用标准细菌学程序进行分析。药敏试验按标准方案采用纸片扩散法进行。使用数据提取表收集人口统计学和细菌学数据。使用SPSS版本22对数据进行清理、输入和分析。结果:总细菌分离率为61.6%(234/380)。革兰氏阳性123株(52.6%)，革兰氏阴性111株(47.4%)。优势菌株为金黄色葡萄球菌100株(42.7%)，其次为大肠杆菌33株(14.1%)、铜绿假单胞菌26株(11.1%)和化脓性葡萄球菌23株(9.8%)。多药耐药(MDR)病原菌占54.3%。其中35株(15.1%)对5种以上药物耐药。革兰氏阴性菌株氨苄西林耐药率最高，为85.9%。革兰氏阳性菌株对红霉素的耐药率最高(31.1%)。金黄色葡萄球菌对青霉素的耐药率为69.7%。结论:单耐药和多重耐药细菌病原菌出现频率较高。因此，在没有培养设施的地区，需要一种替代病原体和抗菌药物敏感性试验监测的方法，以帮助卫生专业人员选择适当的抗生素。[阿比西尼亚人。J.卫生发展。[关键词]伤口感染;细菌分离;药敏分析

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来源期刊

Ethiopian Journal of Health Development PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Ethiopian Journal of Health Development is a multi and interdisciplinary platform that provides space for public health experts in academics, policy and programs to share empirical evidence to contribute to health development agenda. We publish original research articles, reviews, brief communications and commentaries on public health issues, to inform current research, policy and practice in all areas of common interest to the scholars in the field of public health, social sciences and humanities, health practitioners and policy makers. The journal publishes material relevant to any aspect of public health from a wide range of fields: epidemiology, environmental health, health economics, reproductive health, behavioral sciences, nutrition, psychiatry, social pharmacy, medical anthropology, medical sociology, clinical psychology and wide arrays of social sciences and humanities. The journal publishes the following types of contribution: 1) Peer-reviewed original research articles and critical or analytical reviews in any area of social public health. These papers may be up to 3,500 words excluding abstract, tables, and references. Papers below this limit are preferred. 2) Peer-reviewed short reports of research findings on topical issues or published articles of between 2000 and 4000 words. 3) Brief communications, and commentaries debating on particular areas of focus, and published alongside, selected articles. 4) Special Issues bringing together collections of papers on a particular theme, and usually guest edited. 5) Editorial that flags critical issues of public health debate for policy, program and scientific consumption or further debate