Extracellular Biosynthesis of AgNPs by the Bacterium Proteus mirabilis and Its Toxic Effect on Some Aspects of Animal Physiology

纳米粒子(英文) Pub Date : 2014-08-11 DOI:10.4236/ANP.2014.33012

M. Al-harbi, B. El-deeb, N. Mostafa, S. Amer

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引用次数: 12

Abstract

The development of a reliable green chemistry process for the biogenic synthesis of nanomaterials is an important aspect of current nanotechnology research. Silver nanoparticles (AgNPs) have been used as antimicrobial and disinfectant agents. However, there is limited information about its toxicity. Therefore, this study focused on the biosynthesis of AgNPs by the bacterium Proteus mirabilis and on determining its preliminary toxic effect on some aspects of animal physiology. A green method for the synthesis of AgNPs using culture supernatant of Proteus mirabilis has been developed in this study and the synthesized AgNPs were characterized by several techniques. The AgNPs showed a maximum absorbance at 445 nm on ultraviolet-visible spectra. The presence of proteins was identified by Fourier transform-infrared spectroscopy. The reduction of Ag+ to elemental silver was characterized by X-ray spectroscopy analysis. The transmission electron micrograph revealed the formation of polydispersed nanoparticles of 5 - 45 nm. The AgNPs were evaluated for their toxic effect on pregnant female albino rat. The result showed that liver enzymes (AST and ALP) were decreased significantly in the group treated with AgNPs. Mean corpuscular hemoglobin concentration also showed significant increase.

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奇异变形杆菌胞外合成AgNPs及其对动物生理某些方面的毒性作用

开发一种可靠的绿色化学方法来生物合成纳米材料是当前纳米技术研究的一个重要方面。银纳米颗粒(AgNPs)已被用作抗菌和消毒剂。然而，关于其毒性的信息有限。因此，本研究的重点是细菌奇迹变形杆菌(Proteus mirabilis)对AgNPs的生物合成，并确定其对动物生理某些方面的初步毒性作用。本研究开发了一种利用奇异变形杆菌培养上清液合成AgNPs的绿色方法，并通过几种技术对合成的AgNPs进行了表征。AgNPs在紫外可见光谱上的最大吸光度为445 nm。用傅里叶变换红外光谱法鉴定了蛋白质的存在。用x射线光谱分析表征了Ag+还原成单质银的过程。透射电子显微照片显示形成了5 ~ 45nm的多分散纳米颗粒。研究了AgNPs对妊娠雌性白化大鼠的毒性作用。结果表明，AgNPs组肝酶(AST和ALP)显著降低。红细胞血红蛋白平均浓度也明显升高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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纳米粒子(英文)

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