Biosynthesis of monodispersed silver nanoparticles and their activity against Mycobacterium tuberculosis

Abstract

Tuberculosis (TB) is a life threatening disease, caused by Mycobacterium tuberculosis. Emergence of high degree of drug-resistance and prevalence of Mycobacterium other than tuberculosis (MOTT) necessitates the search for new anti-tubercular agents. In that context, silver nanoparticles (AgNPs) which have been known for their antimicrobial activity were evaluated against clinical isolates of M. tuberculosis Multi-Drug Resistant (MDR), Extensive-Drug Resistant (XDR) and MOTT strains, through Microplate Alamar Blue Assay (MABA). All the clinical isolates got inhibited within the MIC range of 6.25 to 12.5 μg/ml of AgNPs. Silver nanoparticles were synthesized by bioreduction of silver nitrate (AgNO3) solution with enzymes extracted from R. stolonifer and characterized using UV-Vis absorption spectrophotometry, FTIR, XRD, AFM. A spherical shaped AgNPs with the average particle size of 5 nm were synthesized and evaluated for their antimycobacterial activity.