Comparison of anxiolytic effects of the homeopathic complex Vita-C 15 in compared with Aconitum Napellus in the acutely stressed C57BL6 mice

Abstract

A phobias and stress are the main mental health problems among the Malaysian population, with global prevalence varying from 8% to 18%. Even so, less than 30% who suffer these disturbances seek treatment. The objective of this study is to evaluate and compare the anxiolytic effects of Aconitum napellus and homeopathic complex Vita-C 15 in the acutely stressed C57BL6 mice by using the fecal corticoid test, open field test (OFT) and c-Fos, NMDAR 2B, NPY 1R and NPY 2R activity through the hippocampus. A double blinded randomized controlled study is conducted at Animal Laboratory of Cyberjaya University College of Medical Sciences (CUCMS). All the animals are acclimatized to constant laboratory conditions for 14 days before starting the experiments. Prior to the experiment, a pilot study is performed to identify the most suitable and ideal potency for the homeopathic remedy of Aconitum napellus. The animals are tested (n=3) per group on the potency of 6C, 30C and 200C. The treatments are carried out over 9 days. 48 male C57BL6 mice (n=6), 4-5 weeks of age are used. They are randomly selected and divided into two groups. Group-1 was the healthy control group of mice which are not exposed to acute stress. Group-2 was the stress group. Comprise of mice expose to acute restraint stress. Prior to restraint stress, the treatments given are Aconitum napellus 30 cH, Homeopathic complex Vita-C 15, Diazepam and placebo. Then the results are evaluated by fecal CORT test and open field test by comparing the anxiolytics between pre-test and post-test. Aconitum napellus 30 cH and Homeopathic complex Vita-C 15 are expected to be more effective and can reduce the occurrence of anxiety in the acutely stressed C57BL6 mice. Thus, research into prevention and supportive therapies is necessary and beneficial for this disorder.