Stability and Efficacy of Anti-dermatophyte Creams From Extract of Kigelia africana Leaves

Alternative & integrative medicine Pub Date : 2018-01-01 DOI:10.4172/2327-5162.1000274

F. Ab, Oyedeji Fo

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引用次数: 0

Abstract

Dermatophytosis are skin infections caused by dermatophytes which can be pathogenic for humans and animals by infecting the stratum corneum, nails, claws or hair and which now affect more than 20%-25% of the world populations especially in the developing countries. Drug resistance and toxicity associated with long-term treatment with existing antifungal drugs necessitate the search for new drugs to treat dermatophytosis. The juice of Kigelia africana leaves has been used by the natives to treat dermatophytosis. The leaves were extracted with water, ethanol and petroleum ether. Phytochemical analyses of the extracts were carried out. Agar disc discussion method was used to determine the antifungal activities against clinical isolates of Microsporum audouinii, Epidermophyton floccosum, Trichophyton mentagrophytes and Malassezia furfur. Herbal creams formulated with 0.5, 1.0 and 2.0 g w/w of the extract were subjected to stability tests using standard methods. FTIR was used to determine if there were new functional groups formed during production of the herbal creams. Sensitivity and efficacy of the products were determined using animal model experiment. The percentage yields of extracts are petroleum ether 6.3%, aqueous 6.5%, and ethanol 7.2%. Percentage ethanol phytochemical composition indicated that for Alkaloid (4.67%), saponins (2.48%), flavonoids (0.81%) and tannins (1.05%). The emulsion produced was an oil-in-water emulsion and had a white colour with pH of 7.02 spread of emulsion, rubbing-in effect and stability to centrifugation was very high. The antifungal results showed that the petroleum ether and aqueous extracts had 10 mm zones of inhibition, while ethanol extract had 15 mm at 10,000 μg/ml against Trichophyton mentagrophytes. Against Microsporum audouinii, the results are petroleum ether and aqueous extracts (10 mm) and ethanol extract (14 mm). Against Epidermophyton floccosum, the results are petroleum ether and aqueous extracts (10 mm) while ethanol was 13 mm. The zones of inhibition recorded by the extracts against Malassezia furfur are aqueous 4 mm, petroleum ether 12 mm and ethanol 15 mm respectively. The results for FTIR showed a spectrum of C-N stretch which peaks at 1019.00 cm-1 for Ka cream. Also, an O-H stretch peak was observed at 3254.00 cm-1. Temperature stability tests carried out indicated increasing stability in the order Ka.water cream

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非洲木叶提取物抗皮肤癣膏的稳定性和疗效

皮肤真菌病是由皮肤真菌引起的皮肤感染，通过感染角质层、指甲、爪子或毛发对人类和动物具有致病性，目前影响着世界上20%-25%以上的人口，特别是在发展中国家。与长期使用现有抗真菌药物治疗相关的耐药性和毒性需要寻找治疗皮肤真菌病的新药。非洲木犀叶的汁液已被当地人用来治疗皮肤癣。用水、乙醇和石油醚对其进行提取。对提取物进行了植物化学分析。采用琼脂盘讨论法测定其对奥杜氏小孢子菌、絮状表皮菌、多植毛癣菌和毛马拉色菌的抑菌活性。用0.5、1.0和2.0 g w/w提取物配制草药乳膏，采用标准方法进行稳定性试验。用红外光谱法测定药膏生产过程中是否有新的官能团形成。通过动物模型实验确定产品的敏感性和有效性。萃取物的收率分别为石油醚6.3%、水6.5%、乙醇7.2%。生物碱(4.67%)、皂苷(2.48%)、黄酮类化合物(0.81%)和单宁(1.05%)分别为乙醇含量。所制得的乳化液为水包油乳化液，呈白色，pH值为7.02，乳化液的扩散、摩擦效果和离心稳定性都很高。结果表明，在1万μg/ml浓度下，石油醚和水提物对红藓毛癣菌的抑制区为10 mm，乙醇提物对红藓毛癣菌的抑制区为15 mm。以石油醚和水提物(10 mm)和乙醇提物(14 mm)对桃小孢子虫进行抑菌试验。对絮凝表皮植物的效果为石油醚和水提物(10 mm)，乙醇(13 mm)。提取液对马拉色菌的抑制区分别为:水抑制区4 mm，石油醚抑制区12 mm，乙醇抑制区15 mm。FTIR结果显示，Ka霜的C-N拉伸谱在1019.00 cm-1处达到峰值。同时，在3254.00 cm-1处观察到O-H拉伸峰。进行的温度稳定性试验表明，稳定性在Ka数量级增加。水霜

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Alternative & integrative medicine

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