Effect of Citrullus colocynthis L. (Cucurbitaceae) agglutinin on gene expression of caspases in Ectomyelois ceratoniae Zeller (Lepidoptera: Crambidae)

Q3 Agricultural and Biological Sciences

Journal of Entomological and Acarological Research Pub Date : 2016-12-19 DOI:10.4081/JEAR.2016.5699

S. Ramzi, A. Sahragard, Jalal Jalali Sendi, A. Aalami

引用次数: 1

Abstract

The larvae of Ectomyelois ceratoniae Zeller were reared on the artificial diets containing 2% of Citrullus colocynthis L. (Cucurbitaceae) lectin and control to find any changes in gene expression of caspases. Specific primers were designed to amplify genes of six found caspases including Caspase 1, 2, 3, 4, 5 and 6. RNA of both treatments were extracted and cDNA molecules were synthesized prior to gene expression. Results of quantitative real-time polymerase chain reaction revealed that expression of all caspases in the fed larvae by 2% of lectin increased in comparison with control except for caspase 1 as 2.99, 5.7, 17.13, 23.18, 10.44-fold. These results are consistent with our previous studies on the lower activities of digestive enzymes that are due to death of midgut cells followed by binding of lectin molecule.

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瓜类凝集素对角裂叶蝉半胱天冬酶基因表达的影响

将ceratoniae ectomyelis Zeller幼虫分别饲喂添加2%瓜科(Citrullus colocynthis L.)凝集素和对照的人工饲料，观察半胱天冬酶基因表达的变化。设计特异性引物扩增已发现的6种Caspase，包括Caspase 1、2、3、4、5和6。在基因表达前提取两种处理的RNA并合成cDNA分子。实时荧光定量聚合酶链反应结果显示，添加2%凝集素后，除caspase 1表达量为对照的2.99倍、5.7倍、17.13倍、23.18倍、10.44倍外，其余caspase的表达量均增加。这些结果与我们之前关于中肠细胞死亡后凝集素分子结合导致消化酶活性降低的研究结果一致。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Entomological and Acarological Research Agricultural and Biological Sciences-Insect Science

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

10 weeks

期刊介绍： The Journal of Entomological and Acarological Research (JEAR), formerly the Bollettino di Zoologia Agraria e di Bachicoltura of the Institute of Entomology of the Università degli Studi, Milano, was founded in 1928 by Remo Grandori. Thereafter, Minos Martelli and Luciano Süss hold the direction of the Journal until December 2011. In January 2012 the Editor decided for the new open-access on-line version of JEAR. The Journal publishes original research papers concerning Arthopods, but reviews, editorials, technical reports, brief notes, conference proceeding, letters to the Editor, book reviews are also welcome. JEAR has four main areas of interest: -Entomology (systematics; morphology; biology; biotechnology; agriculture, ornamental and forest entomology; applied entomology; integrated pest management; biological control; apiculture and apidology; medical, urban and veterinary entomology; etc.) -Stored product pests (biology; integrated pest management; etc.) -Insect Ecology (behaviour; biodiversity; taxonomy; plant insect interaction and ecosystems; biological control; alien species; etc.) -Acarology (systematics; morphology; biology; parasitology; control; etc.) The publication of manuscripts is subject to the approval of the Section Editor who has knowledge of the field discussed in the manuscript in accordance with the principles of Peer Review; referees will be selected from the Editorial Board or among qualified scientists of the international scientific community. Articles must be written in English and must adhere to the guidelines and details contained in the Instructions to Authors.