Identification of dysregulated pathways through SLC30A8 protein interaction in type 1 diabetes mellitus

IF 0.7 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Afreen Bhatty, Z. Rubab, Hafiz Syed Mohammad Osama Jafri, Sheh Zano
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引用次数: 0

Abstract

Objective

The aim of the current study was to explore the gene enrichment and dysregulated pathways on the basis of interaction network analysis of SLC30A8 in type 1 diabetes mellitus (T1DM). SLC30A8 polymorphism could be characterized as a beneficial tool to identify the interacting gene in developing T1DM.

Materials and methods

SLC30A8 interacting protein interaction network was obtained by String Interaction network Version 11.0. Ten proteins were identified interacting with SLC30A8 and were analysed by protein-protein interaction and enrichment network analysis along with Functional Enrichment analysis tool (FunRich 3.1.3) to map the gene data sets. In entire analysis, FunRich database was used as background against all annotated gene/protein list. Protein-protein interaction (PPI) and enrichment network analysis of the selected protein: SLC30A8 gene along with gene mapping and pathway enrichment were performed using FunRich 3.1.3 and String Interaction network Version 11.0.

Results

Biological pathway grouping displayed enriched proteins in TRAIL signalling pathway (p < 0.001). PTPRN, GAD2 and TCF7L2 were enriched in TRAIL Signalling pathway when INS was made focused gene and directly interacting with SLC30A8.

Conclusions

TRAIL signalling pathways were enriched in T1DM. Therefore, SLC30A8 along with PTPRN, GAD2 and TCF7L2 involved in TRAIL pathway must be further explored to understand their in vivo role in T1DM.

通过SLC30A8蛋白相互作用鉴定1型糖尿病的失调途径
目的通过相互作用网络分析SLC30A8基因在1型糖尿病(T1DM)中的富集和失调途径。SLC30A8多态性可作为鉴定T1DM发生过程中相互作用基因的有益工具。材料与方法通过String interaction network Version 11.0获得sslc30a8相互作用蛋白相互作用网络。鉴定出10个与SLC30A8相互作用的蛋白,并利用功能富集分析工具(FunRich 3.1.3)进行蛋白相互作用和富集网络分析,绘制基因数据集。在整个分析中,FunRich数据库作为所有注释基因/蛋白列表的背景。利用FunRich 3.1.3和String interaction network Version 11.0对选取的SLC30A8基因进行蛋白-蛋白相互作用(protein -protein interaction, PPI)和富集网络分析,并进行基因定位和通路富集。结果生物通路分组显示TRAIL信号通路蛋白富集(p < 0.001)。当INS成为聚焦基因并与SLC30A8直接相互作用时,TRAIL信号通路中PTPRN、GAD2和TCF7L2富集。结论T1DM中strail信号通路丰富。因此,SLC30A8与PTPRN、GAD2和TCF7L2参与TRAIL通路还需进一步探索,以了解它们在T1DM中的体内作用。
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来源期刊
AIMS Molecular Science
AIMS Molecular Science BIOCHEMISTRY & MOLECULAR BIOLOGY-
自引率
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发文量
4
审稿时长
5 weeks
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