Detection of honey falsification with an admixture of sodium bicarbonate using the bromothymol blue indicator

I. V. Yatsenko, I. Lotskin, N. Bogatko, V. O. Yevstafyeva, M. Mazanna, М. О. Dehtiarov
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引用次数: 1

Abstract

A method for detecting honey falsification with sodium bicarbonate, added deliberately to reduce the mass fraction of water in honey, increase its density and consistency, eliminate signs of fermentation and reduce contamination by microorganisms, has been substantiated. The used indicator is bromothymol blue alcohol solution with a mass concentration of 0.01% in an amount of 2-3 drops. In the absence of sodium bicarbonate admixture in honey, the colour of the solution is olive; in case of the sodium bicarbonate addition up to 1.0% of the honey volume - it turns light blue; from 1.1 to 2.0% – sky blue; from 2.1 to 3.0% – dark blue. The proposed method is reliable, fast, easy to carry out, does not require a lot of cost for reagents and can be used in combination with other methods for determining the safety and quality of honey, as well as detecting its falsifications. The developed method can be used to detect honey falsification with sodium bicarbonate when determining its safety and quality in production laboratories at facilities for the production of honey and apiproducts, supermarkets, wholesale depots, stores, state laboratories of the State Service of Ukraine on Food Safety and Consumer Protection (SSUFSCP), in government laboratories, in laboratories at designated border inspection posts when carrying out simple laboratory tests using express methods, as well as during the forensic examination of honey. Based on the results of the developed author’s method, it is possible to obtain qualitative characteristics for assessing the honey safety and its quality. The possibility of using an express and effective control system within the framework of simple laboratory tests at designated border inspection posts will allow the implementation of an effective food control system at the stage of their state border verification. Establishing mismatches at the stage of border control and sending laboratory samples for extended laboratory studies (tests) to the state laboratory of the State Service of Ukraine on Food Safety and Consumer Protection, will strengthen the effectiveness of the control system at the stage of early detection of insecurity indicators and prevent the products release that do not meet safety and quality characteristics into circulation. The development of a methodological concept and the creation of a base of methods for detecting honey falsification will contribute to the distribution of safe and high-quality products for consumers, systematization of simple methods for food products study, including honey, in laboratories at designated border inspection posts, during the implementation of state control measures at the Ukrainian state border, will increase the reliability, objectivity and accuracy of the results of the examination, in case of carrying out.
用溴百里酚蓝指示剂检测混合碳酸氢钠伪造蜂蜜
已经证实了一种用碳酸氢钠检测蜂蜜伪造的方法,故意添加碳酸氢钠以降低蜂蜜中水的质量分数,增加其密度和稠度,消除发酵迹象并减少微生物污染。采用质量浓度为0.01%的溴百里酚蓝醇溶液为指示剂,用量为2-3滴。在蜂蜜中不加碳酸氢钠的情况下,溶液呈橄榄色;如果碳酸氢钠的加入量达到蜂蜜体积的1.0%,蜂蜜就会变成浅蓝色;1.1 - 2.0% -天蓝色;从2.1到3.0% -深蓝色。该方法可靠、快速、易于实施,不需要大量的试剂成本,可与其他方法结合使用,用于确定蜂蜜的安全性和质量,以及检测其伪造。所开发的方法可用于在蜂蜜和蜜蜂产品生产设施的生产实验室、超市、批发仓库、商店、乌克兰国家食品安全和消费者保护服务局(SSUFSCP)的国家实验室、政府实验室确定其安全和质量时,检测用碳酸氢钠伪造的蜂蜜。在指定的边境检查站的实验室使用快递方法进行简单的实验室测试时,以及在对蜂蜜进行法医检查时。根据所建立的方法,可以获得蜂蜜安全性和质量评价的定性特征。在指定的边境检查站进行简单实验室测试的框架内使用一种明示和有效的控制系统的可能性,将允许在其国家边境核查阶段实施一种有效的食品控制系统。在边境管制阶段确定不匹配,并将实验室样品送到乌克兰国家食品安全和消费者保护服务局的国家实验室进行长期实验室研究(测试),将加强在早期发现不安全指标阶段控制系统的有效性,并防止不符合安全和质量特征的产品进入流通。方法概念的发展和检测蜂蜜伪造方法的基础将有助于向消费者分发安全和高质量的产品,在指定的边境检查站的实验室中,在乌克兰国家边境实施国家控制措施期间,将食品研究(包括蜂蜜)的简单方法系统化,将提高可靠性。检查结果的客观性和准确性,在情况下进行。
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