NESTED PCR DETECTION OF THEILERIA EQUI INFECTION AND FREQUENCY IN HORSES IMPORTED INTO MEXICO

Journal of Animal and Veterinary Advances Pub Date : 2014-01-01 DOI:10.3923/JAVAA.2014.859.863

M. Valdovinos, C. L. Flores, Jorge Galindo García, D. S. Chiprés, Theodor Duifhuis Rivera, R. A. Estrella, J. Pineda, Javier Germán Rodríguez Carpena

引用次数: 4

Abstract

The purpose of this study was to assess the frequency of Theileria equi in horses imported into Mexico. During different stages of quarantine, 348 blood samples were taken from clinically healthy horses imported into Mexico from 2011 to 2013. Nested PCR (nPCR) of the Merozoite Antigen-1 (EMA-1) gene was performed for pathogen detection. In total, 93 horses tested positive for T. equi resulting in a 26.72% frequency with a 95% Confidence Interval (CI) of 22.07-31.37%. This is the first molecular diagnostics study to identify T. equi-positive horses imported into Mexico these results highlight the importance of nPCR analysis for T. equi in clinically healthy imported horses.

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输入墨西哥马匹马杆菌感染及频率的巢式PCR检测

本研究的目的是评估进口到墨西哥的马中马伊勒菌的频率。在不同的检疫阶段，从2011年至2013年进口到墨西哥的临床健康马匹中采集了348份血液样本。采用Merozoite Antigen-1 (EMA-1)基因巢式PCR (nPCR)检测病原菌。共有93匹马的马弓形虫检测呈阳性，频率为26.72%，95%可信区间(CI)为22.07-31.37%。这是首次对进口到墨西哥的马进行分子诊断研究，这些结果强调了在临床健康的进口马中对马T.进行nPCR分析的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Animal and Veterinary Advances 农林科学-兽医学

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Journal of Animal Veterinary advances is a peer-reviewed, open-access scientific journal which publishes articles related to experiments, treatment, analysis, biological elements and other methods of research connected with veterinary. JAVA started publishing activity in 2002, since that time is updated twice a month, and is available in online and print formats. The publications are reviewed by Editorial Board in accordance with the standards and novelty of the subject, while strictly following ethical guidelines. Subject areas suitable for publication include, but are not limited to the following fields :: Veterinary science :: Animal husbandry :: Animal nutrition :: Anatomy :: Biological science :: Pathology :: Infectious diseases :: Animal physiology :: Animal breeding :: Animal biotechnology :: Transgenic animal production :: Animal parasitology :: Veterinary medicine :: Animal feed and nutrition :: Equine.