Gordonia terrae USTCMS 1066: ITS TAXONOMY, ANTIMICROBIALS AND IMIDAZOLIUM SUSCEPTIBILITY AND EVALUATION AS AN INITIAL SCREENING AGENT FOR ANTI-TB COMPOUNDS AND NATURAL PRODUCTS

Q3 Environmental Science

Philippine Journal of Systematic Biology Pub Date : 2015-02-23 DOI:10.3860/PJSB.V8I0.3328

Carlos P. Garcia, J. Abrantes, Lorna Lioanag, A. Macabeo, E. Quinto

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Abstract

The taxonomic classification and unique characteristics of an air-borne actinomycete exhibiting a dark orange pigmentation were determined by polyphasic analysis. The colony appeared as a contaminant in an agar plate culture of the marine luminous bacterium: Vibrio fischeri USTCMS 1026. Subsequent investigation identified the actinomycete contaminant as MP 1066 for Mycobacterium phlei USTCMS 1066 but was later found to have morphological, physiological, biochemical and genotypic characteristics compatible with member species of the genus Gordonia. BLAST comparison of MP 1066 yielded 100% 16S rDNA sequence alignment with Gordonia terrae so the strain was renamed as Gordonia terrae USTCMS 1066. Its antimicrobial susceptibility profile is similar to that of the pathogenic mycobacteria yielding small MICs and large inhibition zones against the antibiotics: ampicillin (AMP), azithromycin (AZT), amoxicillin-clavulanic acid (AUC), ciprofloxacin (CIP), ethambutol (ETHAM), isoniazid (INH), rifampicin (RIF), tetracycline (TET), sulfamethoxazole – trimethoprim (SXT), and streptomycin (STREP). In addition, the crude ethanolic extracts of Acanthella carteri, Alstonia scholaris Lunasia amara Blanco, Momordica charantia, Wrightia antidysenterica, Zingiber officinale and the pure synthetic imidazole: 1,3-bis(4-methylphenyl) imidazolium chloride likewise inhibited strongly the growth of Gordonia terrae USTCMS 1066. Substances that strongly inhibited Gordonia terrae USTCMS 1066 were also observed to be strongly inhibitory to Mycobacterium tuberculosis indicative of its suitability as an initial assay organism for the screening of anti-TB natural products and synthetic compounds most specially for laboratories that are not equipped with Biosafety Level III facilities. KEYWORDS: Gordonia; Mycobacterium; anti-TB; antibiotics; plant extracts

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地戈登菌的分类、抗菌剂和咪唑的敏感性以及作为抗结核化合物和天然产物的初步筛选剂的评价

采用多相分析方法确定了一种空气传播的暗橙色放线菌的分类和独特特征。在琼脂平板培养的海洋发光细菌:费氏弧菌(Vibrio fischeri) USTCMS 1026中，菌落作为污染物出现。随后的调查确定该放线菌污染物为真菌分枝杆菌USTCMS 1066的mp1066，但后来发现其形态、生理、生化和基因型特征与Gordonia属成员种一致。mp1066的BLAST比对结果显示，该菌株与Gordonia terrae的16S rDNA序列100%一致，因此该菌株更名为Gordonia terrae USTCMS 1066。它的抗菌敏感性与致病性分枝杆菌相似，对氨苄西林(AMP)、阿奇霉素(AZT)、阿莫西林-克拉维酸(AUC)、环丙沙星(CIP)、乙胺丁醇(ETHAM)、异烟肼(INH)、利福平(RIF)、四环素(TET)、磺胺甲恶唑-甲氧苄啶(SXT)和链霉素(STREP)等抗生素产生小的mic和大的抑制区。此外，刺草、桤木、苦参、抗菌白令木、生姜的粗乙醇提取物和纯合成咪唑:1,3-双(4-甲基苯基)咪唑氯也对地Gordonia terrae USTCMS 1066的生长有较强的抑制作用。强烈抑制地戈登菌USTCMS 1066的物质也被观察到对结核分枝杆菌有强烈的抑制作用，这表明它适合作为筛选抗结核天然产物和合成化合物的初始测定生物，特别是对于没有配备生物安全III级设施的实验室。关键词:Gordonia;分枝杆菌;抗结核;抗生素;植物提取物

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来源期刊

Philippine Journal of Systematic Biology Environmental Science-Ecology

CiteScore

1.50

自引率

0.00%

发文量

期刊介绍： The Philippine Journal of Systematic Biology (Print ISSN: 1908-6865; Online ISSN: 2508-0342) is an annual, peer-reviewed journal in English that publishes high quality reports of original research and reviews in the field of Taxonomy & Systematics, Ecology and Conservation Biology. The PJSB is a CHED recognized journal under CHED CMO 50 series of 2017. It is also included in the Clavariate Analysis (formerly a subsidiary of Thomson Reuters) Master Journal List under Zoological Record, BIOSIS Previews and Biological Abstracts.The PJSB is the official publication of the Association of Systematic Biologists of the Philippines (SEC Registration: Association of Philippine Taxonomists, Inc.). The organization financially supports the journal and all its endeavors. The journal aims to build up quality information on animal, plant and microbial diversity in the Philippines. Articles dealing with original research or reviews in Zoological, Botanical or Microbial Systematics, Ecology and Conservation Biology are welcome. Authors are advised to consult a recent issue of PJSB for the current format and style. All manuscripts are reviewed by at least two qualified referees.