Evaluation of the anxiolytic activity of the leaves of Nymphaea lotus (WaterLily) in mice.

Abstract

The anxiolytic effect of the leaves of water lily was investigated using mice as experimental animals. Thirty (30) were mice randomly divided into group 1, 2 and 3. Before the neurobehavioral parameters were assessed, the LD50 and the phytochemical screening of the leaves of the plant were determined. The open field maze and the transition box apparatus were used to determine the level of fear and anxiety related behaviors in mice. In the open field arena, the grooming frequency was statistically not different when compared to control. Stretch attend posture frequency (SAP) was statistically higher in the test groups when compared to control (P<0.001). It was also observed that the frequency of defecation decreased in the treated groups when compared to the control group (P<0.01). The light duration box and frequency of transition were statistically higher (P<0.001) compared to control. Thus, the leaves of the plant tend to reduce the level anxiety in mice.