Geographically Distinct Expression Profile of Host Defense Peptides in the Skin of the Chinese Odorous Frog,Odorrana margaretae

IF 1.2 3区生物学 Q2 ZOOLOGY

Asian Herpetological Research Pub Date : 2013-12-01 DOI:10.3724/SP.J.1245.2013.00288

Guiying, Ling, Li, Jiuxiang, Gao, Hai-ning, Yu, Yipeng, Wang, Jiang, Zhou

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引用次数: 1

Abstract

Odorrana margaretae(Anura: Ranidae) is widely distributed in the southern provinces of China. Previously, 72 antimicrobial peptides(AMPs) belonging to 21 families were identified from the skin of O. margaretae, which were captured in the Hunan province. In the present study, five O. margaretae frogs were captured from the Guizhou province and a total of 28 cDNAs encoding 17 host defense peptides(HDPs) belonging to 14 families were cloned from the skin cDNA library of O. margaretae. Among the 17 HDPs, only one(brevinin-1-Omar5) had been characterized. The distinct HDP expression profiles for O. margaretae in the previous and present study may be attributed to the environmental differences between the sampling locations and the genetic divergence among O. margaretae populations. Besides, 11 of the 17 HDPs identified in the present study were novel for ranids. In order to understand their roles in host defense reactions, three HDPs(odorranain-H-OM1, odorranain-M-OM and ranatuerin-2-OM), which possess low sequence similarity with the known amphibian HDPs, were selected for further chemical synthesis and functional analysis. Odorranain-H-OM1 showed direct antimicrobial activity against bacteria and fungi. Odorranain-M-OM exhibited concentration-dependent anti-oxidant activity. Ranatuerin-2-OM showed lectin-like activity and could strongly hemagglu-tinate human intact erythrocytes with or without the presence of Ca2+. The diverse activities of HDPs implied that they may play different roles in host defense reactions of O. margaretae.

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中国臭蛙皮肤宿主防御肽的地理差异表达谱

马臭蛛(无尾目:臭蛛科)广泛分布于中国南方省份。此前，从湖南省采集的麻豆皮中分离到21个科的72个抗菌肽。本研究从贵州捕获5只姬蛙，从姬蛙皮肤cDNA文库中克隆了14科17个宿主防御肽(host defense peptide, HDPs)的28个cDNA。在17个HDPs中，仅鉴定出1个(brevinin-1-Omar5)。以往和本研究中黄花草HDP表达谱的差异可能与不同取样地点的环境差异和黄花草居群间遗传差异有关。此外，本研究中鉴定的17个HDPs中有11个是ranids的新HDPs。为了了解它们在宿主防御反应中的作用，我们选择了与已知两栖动物HDPs序列相似性较低的3种HDPs(odorranain-H-OM1、odorranain-M-OM和ranatuerin-2-OM)进行化学合成和功能分析。Odorranain-H-OM1对细菌和真菌具有直接抑菌活性。Odorranain-M-OM具有浓度依赖性的抗氧化活性。Ranatuerin-2-OM具有凝集素样活性，无论是否存在Ca2+，都能对人完整红细胞进行强血凝。HDPs活性的多样性表明它们可能在黄花蓟马的宿主防御反应中发挥不同的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asian Herpetological Research ZOOLOGY-

CiteScore

2.80

自引率

7.10%

发文量

441

审稿时长

>12 weeks

期刊介绍： AHR aims to provide a forum for herpetologists and related scientists interested in conducting international academic exchanges and joint studies, and a platform for introducing their newly made scientific and technological data, and publishing their research results and achievements in the world, but focusing on the Asian-Pacific Region. The principal criteria of AHR for acceptance of articles for publication are the quality and significance of the research, breadth of interest of the work to the readership, and the clarity and effectiveness of communication. AHR welcomes submission of manuscripts from authors in all countries of the world, though with a focus on the herpetological studies in the Asian and Pacific Region, including major articles, shorter communications and review articles.