Development and Characterization of 15 Polymorphic Microsatellite Markers for a Highly Adaptive and Wide-range Frog (Microhyla fissipes)

IF 1.2 3区生物学 Q2 ZOOLOGY

Asian Herpetological Research Pub Date : 2013-09-01 DOI:10.3724/sp.j.1245.2013.00202

J. Zhang, J. Wang, J. Jiang

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Abstract

In order to analyze population genetic structure at multiple spatial scales, microsatellite loci were developed for the ornamented pygmy frog (Microhyla fisszpes), and 15 polymorphic microsatellite loci were successfully screened from 105 individuals, of which 82 from four populations distributed in the Sichuan Basin and 23 from the Sangzhi population in western Hunan. Five loci were found to deviate significantly from Hardy-Weinberg equilibrium in one to three populations, probably due to small sample size or null alleles. The average number of alleles in all loci was 8.5, ranging from 4 to 13, and the observed and expected heterozygosity ranged from 0.26 to 0.90 and 0.63 to 0.90, respectively. The Sangzhi population and the remaining four populations can be clearly separated using Bayesian clustering methods, showing that the genetic structure of M fissipes was probably affected by the topography, especially mountain barriers. These polymorphic microsatellite loci could be used for further study on the landscape genetics of this highly adaptive and widely distributed species.

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高适应性大范围蛙(Microhyla fissipes) 15个多态微卫星标记的开发与鉴定

为了分析多空间尺度上的种群遗传结构，对纹饰侏儒蛙(Microhyla fisszpes)进行了微卫星定位，从105个个体中成功筛选到15个多态性微卫星位点，其中82个来自四川盆地4个种群，23个来自湘西桑植种群。在1 ~ 3个种群中，有5个位点明显偏离Hardy-Weinberg平衡，这可能是由于样本量小或等位基因缺失所致。所有位点的平均等位基因数为8.5个，范围为4 ~ 13个，观察杂合度和预期杂合度分别为0.26 ~ 0.90和0.63 ~ 0.90。利用贝叶斯聚类方法可以将桑植居群与其余4个居群明显区分开，表明M裂种的遗传结构可能受到地形，特别是山地屏障的影响。这些多态微卫星位点可为进一步研究这种高适应性、分布广泛的物种的景观遗传学提供依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asian Herpetological Research ZOOLOGY-

CiteScore

2.80

自引率

7.10%

发文量

441

审稿时长

>12 weeks

期刊介绍： AHR aims to provide a forum for herpetologists and related scientists interested in conducting international academic exchanges and joint studies, and a platform for introducing their newly made scientific and technological data, and publishing their research results and achievements in the world, but focusing on the Asian-Pacific Region. The principal criteria of AHR for acceptance of articles for publication are the quality and significance of the research, breadth of interest of the work to the readership, and the clarity and effectiveness of communication. AHR welcomes submission of manuscripts from authors in all countries of the world, though with a focus on the herpetological studies in the Asian and Pacific Region, including major articles, shorter communications and review articles.

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