Serum level of miR-126 in patients with hypertensive intracerebral hemorrhage and its clinical significance

中国基层医药 Pub Date : 2020-04-01 DOI:10.3760/CMA.J.ISSN.1008-6706.2020.07.008

Haiming Chen, D. Dong, Xian-Dong Wang

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Abstract

Objective To investigate the serum level of miR-126 in patients with hypertensive intracerebral hemorrhage and to elucidate its clinical significance in hypertensive cerebral hemorrhage. Methods From January 2015 to December 2018, 60 patients with hypertensive cerebral hemorrhage were selected as observation group, and 60 healthy subjects were selected as control group in the People's Hospital of Lin'an Affiliated to Hangzhou Medical College.The serum levels of miR-126 were determined by reverse transcription-polymerase chain reaction (RT-PCR). The serum levels of C-reactive protein (CRP), tumor necrosis factor-ɑ (TNF-ɑ) and nuclear factor kappa B (NF-κB) were measured by enzyme-linked immunosorbent assay (ELISA). Results The serum level of miR-126 in the observation group was lower than that in the control group [(0.46±0.11) vs.(1.00±0.13), t=24.562, P<0.05], and the serum levels of CRP, TNF-ɑ and NF-κB were higher than those in the control group[(8.74±0.69)mg/L vs.(3.98±0.61)mg/L, (62.43±8.26)μg/L vs.(31.28±6.54)μg/L, (19.73±1.02)μmol/L vs.(4.92±0.87)μmol/L, t=40.034, 22.902, 85.570, all P<0.05]. There were statistically significant differences in serum miR-126, CRP, TNF-ɑ, NF-κB levels among patients with different severity of hypertensive intracerebral hemorrhage[(0.57±0.09) vs.(0.44±0.12) vs.(0.36±0.11), (6.91±0.72)mg/L vs.(8.63±0.67)mg/L vs.(9.14±0.75)mg/L, (53.16±8.19)μg/L vs.(62.57±8.33)μg/L vs.(70.38±8.09)μg/L, (12.49±0.97)μmol/L vs.(19.58±1.11)μmol/L vs.(25.64±1.05)μmol/L, F=14.433, 41.379, 17.796, 623.893, all P<0.05], with the severity increased, the serum miR-126 level decreased, serum CRP, TNF-ɑ and NF-κB levels increased.The serum miR-126 level in patients with hypertensive cerebral hemorrhage of the poor prognosis group was lower than that of the good prognosis group [(0.53±0.10) vs.(0.38±0.12), t=5.279, P<0.05], and the serum levels of CRP, TNF-ɑ and NF-κB in the poor prognosis group were higher than those in the good prognosis group[(7.85±0.64)mg/L vs.(9.16±0.73)mg/L, (51.07±8.32)μg/L vs.(73.26±8.17)μg/L, (14.73±1.08)μmol/L vs.(26.52±0.99)μmol/L, t=7.392, 10.317, 43.424, all P<0.05]. The area of brain edema in patients with hypertensive intracerebral hemorrhage was negatively correlated with serum miR-126 (r=-0.623, P<0.05), and positively correlated with serum CRP, TNF-ɑ and NF-κB (r=0.581, 0.618, 0.642, all P<0.05). The serum miR-126 level was negatively correlated with CRP, TNF-ɑ and NF-κB in patients with hypertensive intracerebral hemorrhage (r=-0.593, -0.624, -0.618, all P<0.05). Conclusion The serum level of miR-126 is reduced in patients with hypertensive intracerebral hemorrhage, which may participate in the pathogenesis of hypertensive cerebral hemorrhage by participating in inflammatory reaction.The detection of serum miR-126 level has great value in evaluation of the severity and prognosis of hypertensive cerebral hemorrhage. Key words: Intracranial hemorrhage, hypertensive; MicroRNAs; C-reactive protein; Tumor necrosis factor-alpha; Nuclear transcription factor κB

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高血压脑出血患者血清miR-126水平及其临床意义

目的探讨高血压脑出血患者血清miR-126水平，探讨其在高血压脑出血中的临床意义。方法选取2015年1月~ 2018年12月杭州医学院附属临安人民医院高血压脑出血患者60例作为观察组，健康人群60例作为对照组。采用逆转录聚合酶链反应(RT-PCR)检测血清miR-126水平。采用酶联免疫吸附法(ELISA)检测血清c反应蛋白(CRP)、肿瘤坏死因子- α (TNF- α)、核因子κB (NF-κB)水平。结果观察组患者血清miR-126水平低于对照组[(0.46±0.11)比(1.00±0.13)，t=24.562, P<0.05]，血清CRP、TNF- β、NF-κ b水平高于对照组[(8.74±0.69)mg/L比(3.98±0.61)mg/L，(62.43±8.26)μmol/L比(31.28±6.54)μmol/L，(19.73±1.02)μmol/L比(4.92±0.87)μmol/L, t=40.034、22.902、85.570,P<0.05]。有统计上显著的差异在血清mir - 126、c反应蛋白、肿瘤坏死因子-ɑ,NF -κB水平不同程度的高血压脑出血患者[(0.57±0.09)和(0.44±0.12)和(0.36±0.11),(6.91±0.72)mg / L和(8.63±0.67)mg / L和(9.14±0.75)mg / L(53.16±8.19)μg / L和(62.57±8.33)μg / L和(70.38±8.09)μg / L,(12.49±0.97)μmol / L和(19.58±1.11)μmol / L和(25.64±1.05)μmol / L F = 14.433, 41.379, 17.796, 623.893, P < 0.05),与严重程度增加,血清miR-126水平降低，血清CRP、TNF- α、NF-κ b水平升高。血清mir - 126水平的高血压性脑出血患者预后不良组低于预后良好组((0.53±0.10)和(0.38±0.12),t = 5.279, P < 0.05),血清CRP水平,肿瘤坏死因子-ɑ和NF -κB的预后不良组高于预后良好组(mg / L(7.85±0.64)和(9.16±0.73)mg / L(51.07±8.32)μg / L和(73.26±8.17)μg / L,(14.73±1.08)μmol / L和(26.52±0.99)μmol / L、t = 7.392, 10.317, 43.424, P < 0.05)。高血压脑出血患者脑水肿面积与血清miR-126呈负相关(r=-0.623, P<0.05)，与血清CRP、TNF- α、NF-κ b呈正相关(r=0.581、0.618、0.642，均P<0.05)。高血压脑出血患者血清miR-126水平与CRP、TNF- α、NF-κ b呈负相关(r=-0.593、-0.624、-0.618,P均<0.05)。结论高血压脑出血患者血清miR-126水平降低，可能通过参与炎症反应参与高血压脑出血的发病机制。血清miR-126水平的检测对评价高血压脑出血的严重程度及预后有重要价值。关键词:颅内出血;高血压;小分子核糖核酸;c反应蛋白;肿瘤坏死因子;核转录因子κB

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来源期刊

中国基层医药

CiteScore

0.10

自引率

0.00%

发文量

32251

期刊介绍： Since its inception, the journal "Chinese Primary Medicine" has adhered to the development strategy of "based in China, serving the grassroots, and facing the world" as its publishing concept, reporting a large amount of the latest medical information at home and abroad, prospering the academic field of primary medicine, and is praised by readers as a medical encyclopedia that updates knowledge. It is a core journal in China's medical and health field, and its influence index (CI) ranks Q2 in China's academic journals in 2022. It was included in the American Chemical Abstracts in 2008, the World Health Organization Western Pacific Regional Medical Index (WPRIM) in 2009, and the Japan Science and Technology Agency Database (JST) and Scopus Database in 2018, and was included in the Wanfang Data-China Digital Journal Group and the China Academic Journal Comprehensive Evaluation Database.