Effects of rhEPO on oxidative stress levels and endothelial cell apoptosis of patients with uremic serum

Abstract

Objective Effects of rhEPO on oxidative stress levels and endothelial cell apoptosis of patients with uremic serum. Methods From January 2015 to January 2016, 9 cases patients with uremic serum from the Third Affiliated of Xinxiang Medical Hospital and 9 cases of healthy volunteers were selected in this study. According to the effect of different methods to HUVECs, HUVECs were divided into 3 groups: control group (containing 10% health serum medium), uremia group (containing 10% uremic patients serum medium), rhEPO group (medium containing 5, 10, 15 U/ml three different concentrations of rhEPO and 10% uremic patients serum medium medium) and 10% of patients with uremia. After Each medium of group culture effecting on endothelial cells 24 h, reactive oxygen species were detected by colorimetry and cell apoptosis was evaluated by TUNEL assay. Results rhEPO could reduce oxidative stress levels in cells, and had significant inhibition against apoptosis of endothelial cells induced by uremia serum (P<0.05), in addition, when the concentration of rhEPO increases, the oxidative stress levels and endothelial cell apoptosis index were reduced gradually (P<0.05). The results of variance correlation analysis showed that the oxidative stress levels and endothelial cell apoptosis index were obviously positive correlation relations (r=0.89, P<0.01). Conclusion rhEPO could significantly inhibit the uremia serum induced apoptosis of endothelial cells, the mechanism may be related to the rhEPO which can reduce the oxidative stress reaction. Key words: Recombinant human erythropoietin; Uremic; Oxidative stress levels; Endothelial cells; Colorimetry