Isolation of antibacterial peptides with low molecular weight from the spleen of Anguilla japonica

Q4 Environmental Science

水产学报 Pub Date : 2013-01-01 DOI:10.3724/SP.J.1231.2013.38528

Ying-jiao Liang, R. Guan, Wen-shu Huang

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Abstract

The acetic acid extract of the spleen from Japanese eel,Anguilla japonica,was separated by tangential flow ultrafiltration with 10 and 3 ku fibre columns,and the two protein samples of the molecular weight(Mw)10 ku and the Mw3 ku were purified by using the cationic exchange liquid chromatography together with reverse-phase high-performance liquid chromatography(RP-HPLC).The results showed that three compositions(the protein samples of Mw10 ku,Mw10 ku and Mw3 ku)were separated from the acid extract with two fibre columns.The samples of Mw10 ku and Mw3 ku exhibited stronger antibacterial activity than the protein sample of the Mw10 ku.The protein sample with the Mw10 ku was purified by the cationic exchange liquid chromatography and using pH 9 buffer solution in RP-HPLC,and we obtained an antibacterial peptide with the molecular weight of 1 391.82 u when determined by MALDI-TOF MS.The pH 9 and the pH 2 buffer solutions were applied in RP-HPLC of the factions obtained from cationic exchange liquid chromatography of the protein sample with the Mw3 ku,and we also obtained an antibacterial peptide with the molecular weight of 839.19 u when determined by MALDI-TOF MS.This study suggests that the main antibacterial components in the spleen of A.japonica are the proteins of low molecular weight.

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日本鳗鲡脾脏低分子量抗菌肽的分离

采用10和3 ku纤维柱切向流超滤分离日本鳗鲡脾脏乙酸提取物，并采用阳离子交换液相色谱和反相高效液相色谱(RP-HPLC)对分子量为10 ku和Mw3 ku的两种蛋白质样品进行纯化。结果表明，用两根纤维柱从酸提物中分离出了Mw10 ku、Mw10 ku和Mw3 ku蛋白样品。Mw10 ku和Mw3 ku蛋白样品的抑菌活性高于Mw10 ku蛋白样品。Mw10 ku的蛋白质样品由阳离子交换液相色谱纯化产物和使用9缓冲溶液pH值,我们获得一个抗菌肽的分子量1 391.82 u时由MALDI-TOF MS.The酸碱9和2缓冲派系获得的解决方案应用于rp -阳离子交换液相色谱的蛋白质样品Mw3 ku,我们也获得了抗菌肽的分子量MALDI-TOF ms测定值为839.19 u，提示粳稻脾脏中主要的抑菌成分为低分子量蛋白。

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来源期刊

水产学报 Environmental Science-Management, Monitoring, Policy and Law

CiteScore

1.40

自引率

0.00%

发文量

5213

期刊介绍： "Fisheries of" mainly reflects the results of scientific research and development of the direction of aquaculture for domestic and foreign academic exchanges Fisheries Service. Mainly basic research published in Fisheries, aquaculture and proliferation of fishing waters environmental protection, preservation of aquatic products processing and utilization, fishing equipment, and other aspects of mechanical papers, research briefings and reviewed.