A fluorescent staining method for marking the cuttlefish(Sepiella japonica)

Q4 Environmental Science

水产学报 Pub Date : 2013-01-01 DOI:10.3724/SP.J.1231.2013.38443

Jun Liang, Weiding Wang, Hanxiang Xu, Yong-dong Zhou, Kaida Xu

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Abstract

It is necessary to establish a highly efficient method of marking,which will provide technical guarantee for a more accurate assessment of stock-enhancement effect of the cuttlefish(Sepiella japonica).The cuttlefish were chosen as the experimental material in this study.The larval cuttlefish were immersed in alizarin complexone(ALC)to dye the cuttlebone.The experiment was divided into 5 groups with concentration gradient and 2 time periods,and doral mantle length,body weight and survival rate of cuttlefish were measured and observed.The trial group and control group(50 ind/group)were reared in 2.5 m diameter circular concrete ponds,respectively.The cuttlefish were sampled randomly in order to examine the dye-retention of the tagged cuttlebone after being tagged 5,12,19,26 d.Moreover,the tagged cuttlefish were examined at high temperature treatment for 5 min.The results showed that the optimised dye concentration and immerged time were 90 mg/L and 24 h,respectively.The doral mantle length of cuttlefish had a significant positive correlation with body weight(P0.01),and there was no significant difference in growth between trial group and control group(P0.05).The tagged oval pink ring was still clear in the cuttlebone even after being tagged 26 d.The range and color of tagging parts were not changed before and after high temperature treatment.Additionally,the proposed method has effect on both internal signs and external signs,and the survival rate and dye-retention rate of the tagged cuttlefish are 100%.It is concluded that this method can be successfully applied to marking cuttlefish,and offer a number of benefits,including simple,efficient,high sample throughput,and a wide range of application prospects.

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墨鱼荧光染色标记方法的研究

有必要建立一种高效的标记方法，为更准确地评价墨鱼的增库效果提供技术保障。本研究选用墨鱼作为实验材料。用茜素络合物(ALC)浸泡墨鱼幼鱼，对墨骨进行染色。试验按浓度梯度和2个时间段分为5组，测量和观察墨鱼的背地幔长度、体重和存活率。试验组和对照组(50尾/组)分别饲养在直径2.5 m的圆形混凝土池中。随机抽取墨鱼，分别在5、12、19、26 d后检测标记墨鱼的染料保留率，并对标记后的墨鱼进行5 min的高温处理，结果表明，最佳的染料浓度和浸泡时间分别为90 mg/L和24 h。墨鱼的背地幔长度与体重呈极显著正相关(P0.01)，试验组与对照组的生长差异不显著(P0.05)。标记的椭圆形粉红色环在标记26 d后仍然清晰，标记部位的范围和颜色在高温处理前后没有变化。此外，该方法对内部标志和外部标志都有影响，标记后的墨鱼存活率和染料保留率均为100%。结果表明，该方法可成功应用于墨鱼的标记，具有简单、高效、样品通量高的优点，具有广泛的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

水产学报 Environmental Science-Management, Monitoring, Policy and Law

CiteScore

1.40

自引率

0.00%

发文量

5213

期刊介绍： "Fisheries of" mainly reflects the results of scientific research and development of the direction of aquaculture for domestic and foreign academic exchanges Fisheries Service. Mainly basic research published in Fisheries, aquaculture and proliferation of fishing waters environmental protection, preservation of aquatic products processing and utilization, fishing equipment, and other aspects of mechanical papers, research briefings and reviewed.