Isolation and identification of Bacillus sp.and evaluation of its effect on WSSV disease resistance in Litopenaeus vannamei

Q4 Environmental Science

水产学报 Pub Date : 2013-01-01 DOI:10.3724/SP.J.1231.2013.38373

Yan Sun, Xiaoling Song, Fei Liu, Yuhong Li, Jie Huang

{"title":"Isolation and identification of Bacillus sp.and evaluation of its effect on WSSV disease resistance in Litopenaeus vannamei","authors":"Yan Sun, Xiaoling Song, Fei Liu, Yuhong Li, Jie Huang","doi":"10.3724/SP.J.1231.2013.38373","DOIUrl":null,"url":null,"abstract":"In order to select WSSV disease-resistant strains,a marine Bacillus sp.was isolated and purified from digestive tract from the healthy of Chinese shrimp(Fenneropenaeus chinensis).The morphology and gram stain indicated that the strain is a gram-positive and rod-shaped bacterium,with a single polar flagellum and oval spores.The colony is circular and slightly raised.Identification analyses by the Biolog Carbon Source Utilization,ATB Microbial Identification System,and the fatty acid gas chromatography indicated that the most similar strain in physiological and biochemical characteristics is Bacillus firmus.Phylogenetic analysis with 16S rRNA sequence showed that it has 100% homology with the previously reported Bacillus firmus.The cultured strain PC024 was added to feed by conglutinating to the surface of the pellets and fed to Litopenaeus vannamei.After feeding for 20 d,the shrimp was challenged with WSSV by intramuscular injection to observe the cumulative mortality in 14 d post-challenge.The results showed that the experimental group fed with the strain PC024 had a relative survival rate of 33.7% in comparison with the control group.The immune-related enzyme activity in the serum and hepatopancreas of shrimp in the experimental group was significantly increased than the control group.And the total number of bacteria of the intestine of the experimental group is always significantly higher than that of the control group and Bacillus firmus can be isolated from the experimental group.This study suggests that the Bacillus firmus PC024 can be used as the WSSV disease prevention probiotic strains and can further be used in shrimp farming.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"574"},"PeriodicalIF":0.0000,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"水产学报","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.3724/SP.J.1231.2013.38373","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Environmental Science","Score":null,"Total":0}

引用次数: 4

Abstract

In order to select WSSV disease-resistant strains,a marine Bacillus sp.was isolated and purified from digestive tract from the healthy of Chinese shrimp(Fenneropenaeus chinensis).The morphology and gram stain indicated that the strain is a gram-positive and rod-shaped bacterium,with a single polar flagellum and oval spores.The colony is circular and slightly raised.Identification analyses by the Biolog Carbon Source Utilization,ATB Microbial Identification System,and the fatty acid gas chromatography indicated that the most similar strain in physiological and biochemical characteristics is Bacillus firmus.Phylogenetic analysis with 16S rRNA sequence showed that it has 100% homology with the previously reported Bacillus firmus.The cultured strain PC024 was added to feed by conglutinating to the surface of the pellets and fed to Litopenaeus vannamei.After feeding for 20 d,the shrimp was challenged with WSSV by intramuscular injection to observe the cumulative mortality in 14 d post-challenge.The results showed that the experimental group fed with the strain PC024 had a relative survival rate of 33.7% in comparison with the control group.The immune-related enzyme activity in the serum and hepatopancreas of shrimp in the experimental group was significantly increased than the control group.And the total number of bacteria of the intestine of the experimental group is always significantly higher than that of the control group and Bacillus firmus can be isolated from the experimental group.This study suggests that the Bacillus firmus PC024 can be used as the WSSV disease prevention probiotic strains and can further be used in shrimp farming.

查看原文本刊更多论文

凡纳滨对虾芽孢杆菌的分离鉴定及其对WSSV病抗性的评价

为了筛选WSSV抗病菌株，从健康中国对虾(Fenneropenaeus chinensis)消化道中分离纯化了一株海洋芽孢杆菌。革兰氏染色结果表明该菌株为革兰氏阳性菌，棒状，单极鞭毛，卵圆形孢子。蜂群呈圆形，略凸起。采用生物碳源利用、ATB微生物鉴定系统和脂肪酸气相色谱法进行鉴定分析，结果表明，在生理生化特征上最相似的菌株是硬芽孢杆菌。16S rRNA序列的系统发育分析表明，该菌株与先前报道的芽孢杆菌具有100%的同源性。将培养的菌株PC024粘附在微球表面，加入饲料中，喂给凡纳滨对虾。饲养20 d后，肌肉注射WSSV攻毒，观察攻毒后14 d的累积死亡率。结果表明，试验组与对照组相比，PC024菌株的相对存活率为33.7%。试验组对虾血清和肝胰脏中免疫相关酶活性显著高于对照组。试验组的肠道细菌总数始终显著高于对照组，并可从试验组中分离出硬芽孢杆菌。本研究表明，硬芽孢杆菌PC024可作为WSSV病防治益生菌菌株，进一步应用于对虾养殖。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

水产学报 Environmental Science-Management, Monitoring, Policy and Law

CiteScore

1.40

自引率

0.00%

发文量

5213

期刊介绍： "Fisheries of" mainly reflects the results of scientific research and development of the direction of aquaculture for domestic and foreign academic exchanges Fisheries Service. Mainly basic research published in Fisheries, aquaculture and proliferation of fishing waters environmental protection, preservation of aquatic products processing and utilization, fishing equipment, and other aspects of mechanical papers, research briefings and reviewed.