Identification of type I microsatellite markers and their polymorphism in Portunus trituberculatus

Q4 Environmental Science

水产学报 Pub Date : 2013-01-01 DOI:10.3724/SP.J.1231.2013.38343

Jianjian Lü, Yu Wang, B. Gao, Jian Li, Ping Liu

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引用次数: 1

Abstract

In the present study,typeⅠmicrosatellites of Portunus trituberculatus were screened from 13 985 ESTs in the NCBI database by bioinformatics approach.A total of 287 type Ⅰ microsatellites were identified,among which 173 were the type of dinucleotide repeat being the most abundant microsatellite and accounting for 60.3% of the identified microsatellite markers,and 79 were the type of trinucleotide accounting for 27.5%,while hexa-,penta-and tetra-nucleotide repeats were rare.Among the dinucleotide repeats,AC/GT was the most abundant type with percentage of 53.8%,followed by the AG/CT type with 37.0%,while AT and GC were rare.14 microsatellites were selected for polymorphism analyses,8 of them showed polymorphism in a wild population of P.trituberculatus.The mean PIC and heterozygosity values were 0.57 and 0.63,respectively.Among them,6 PIC of microsatellites greater than 0.5,showing high polymorphism.This study showed that the way of screening typeⅠmicrosatellite markers from EST data was feasible.These typeⅠmicrosatellites can be used for further study in genetic diversity,genetic mapping and quantitative trait loci(QTLs)identification in P.trituberculatus.

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三瘤梭子鱼I型微卫星标记及其多态性鉴定

本研究采用生物信息学方法，从NCBI数据库中的13 985条est中筛选了三瘤波图努斯Ⅰ型微卫星。共鉴定出287个Ⅰ型微卫星，其中二核苷酸重复型微卫星最多173个，占鉴定微卫星标记的60.3%，三核苷酸重复型微卫星79个，占鉴定微卫星标记的27.5%，而六、五、四核苷酸重复型微卫星较少。在二核苷酸重复序列中，AC/GT型最多，占53.8%，AG/CT型次之，占37.0%，AT和GC较少。选取14个微卫星进行多态性分析，其中8个微卫星在三瘤假单胞菌野生居群中显示多态性，平均PIC值为0.57，杂合度为0.63。其中，6颗微卫星的PIC大于0.5，表现出较高的多态性。本研究表明，从EST数据中筛选Ⅰ型微卫星标记物的方法是可行的。这些Ⅰ型微卫星可用于三管棘球蚴遗传多样性、遗传作图和数量性状位点(qtl)鉴定的进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

水产学报 Environmental Science-Management, Monitoring, Policy and Law

CiteScore

1.40

自引率

0.00%

发文量

5213

期刊介绍： "Fisheries of" mainly reflects the results of scientific research and development of the direction of aquaculture for domestic and foreign academic exchanges Fisheries Service. Mainly basic research published in Fisheries, aquaculture and proliferation of fishing waters environmental protection, preservation of aquatic products processing and utilization, fishing equipment, and other aspects of mechanical papers, research briefings and reviewed.