Effects of different inoculation densities on growth and toxin production of Alexandrium minutum

Q4 Environmental Science

Journal of Fisheries of China Pub Date : 2013-01-01 DOI:10.3724/SP.J.1231.2013.38266

Zhongyuan Bian, Xihong Yang, Wancui Xie, Chaohua Zhang, Zhuo Li, Yadong Yang

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引用次数: 1

Abstract

Effects of different inoculation densities on growth and toxin production of Alexandrium minutum were studied.Experiments were conducted under conditions of varying inoculation densities(0.05×104,0.10×104,0.15×104,0.30×104 cells/mL).Higher inoculation densities always resulted in shorter lag phase of A.minutum,and an earlier setting in of the steady growth phase.However,the specific growth rate and maximum cell concentration of A.minutum decreased with increasing inoculation densities.The multiplication models developed correlated well with the observations,as the inoculation density increased,the initial-density dependent parameter a also deceased and the reduction of environmental capacity,instantaneous multiplication included.According to MBA analysis,toxicity of A.minutum is typically highest when cells are growing fast in early exponential phase and lowest as growth slows and eventually stops in plateau phase.The PSP-toxin congeners,GTX1(2.14 fmol/cell),GTX2(2.08 fmol/cell),GTX3(4.97 fmol/cell),GTX4(5.04 fmol/cell)were analysed by high-performance liquid chromatography with fluorescence diction(HPLC-FLD).Result of this study implied that toxin production of the dinoflagellate A.minutum is better in 0.10×104-0.15×104 cells/mL than other condition of inoculations densities.

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不同接种密度对亚历山大菌生长和产毒的影响

研究了不同接种密度对亚历山大菌生长和产毒的影响。实验在不同接种密度(0.05×104,0.10×104,0.15×104,0.30×104 cells/mL)条件下进行。接种密度越高，黄芪滞生期越短，稳生期越早。但随着接种密度的增加，小黄菌的特定生长率和最大细胞浓度呈下降趋势。所建立的繁殖模型与观测结果吻合较好，随着接种密度的增加，初始密度依赖参数a也随之消失，环境容量也随之减少，其中包括瞬时繁殖。根据MBA分析，在早期指数期细胞快速生长时，毒力最高，在缓慢生长并最终停止生长的平台期毒性最低。采用高效液相色谱-荧光法(HPLC-FLD)对psp毒素同源基因GTX1(2.14 fmol/cell)、GTX2(2.08 fmol/cell)、GTX3(4.97 fmol/cell)、GTX4(5.04 fmol/cell)进行分析。本研究结果表明，0.10×104-0.15×104细胞/mL接种密度下，鞭毛藻的产毒效果优于其他接种密度。

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来源期刊

Journal of Fisheries of China Environmental Science-Management, Monitoring, Policy and Law

CiteScore

1.40

自引率

0.00%

发文量

5213

期刊介绍： "Fisheries of" mainly reflects the results of scientific research and development of the direction of aquaculture for domestic and foreign academic exchanges Fisheries Service. Mainly basic research published in Fisheries, aquaculture and proliferation of fishing waters environmental protection, preservation of aquatic products processing and utilization, fishing equipment, and other aspects of mechanical papers, research briefings and reviewed.