Target gene expression and morphological masculinization in mosquitofish(Gambusia affinis)exposed to 17α-methyltestosterone

Q4 Environmental Science

水产学报 Pub Date : 2013-01-01 DOI:10.3724/SP.J.1231.2013.38212

Junjie Fan, Shao-Zhong Xu, Z. Fang, Rushu Wen

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引用次数: 1

Abstract

The effects of 17α-methyltestosterone(MT)on target gene expression and morphological masculinization in female mosquitofish(Gambusia affinis)during the growth and development is studied in order to test if the level of ARα mRNA expression in mosquitofish can be considered a valid biomarker for aquaculture androgen POPs.With juvenile female mosquitofish exposed to MT with 0.5,5,50 and 500 nmol/L for 21 d,the body length(BL),body weight(BW),body health index(CF),and the anal fin 3rd fin section length(FL),the section number(FJ),and the most wide department width(FW),as well as the level of ARα mRNA and VTGα mRNA expression were quantitatively determined by morphology and in vitro methods,and set the control group and the parallel groups.The results showed that the BL and CF in female juvenile exposed to different concentrations of MT for 21 d were not significantly different,only the BW in fish exposed to 50 and 500 nmol/L of high-dose groups was significantly decreased(P0.05)when compared with the control group;the FJ in 3rd fin of anal fin showed a significant increase,FL was along with extending and FW also showed a significant increase in width(P0.05);Female juveniles were exposed to different concentrations of MT after 21 d,ARα mRNA expression level was displayed dose-related increase significantly(P0.05).The results indicated that the androgenic effects of MT was very significant,resulting in the morphological masculinization in female mosquitofish during the growth and development process;ARα mRNA expression levels could be used as the ideal biomarkers for monitoring the aquaculture water androgen pollutants.

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17α-甲基睾酮对食蚊鱼靶基因表达及形态雄性化的影响

本文研究了17α-甲基睾酮(MT)对雌性食蚊鱼(Gambusia affinis)生长发育过程中靶基因表达和形态雄性化的影响，以验证ARα mRNA表达水平是否可以作为水产养殖雄激素POPs的有效生物标志物。用0.5、5、50和500 nmol/L MT处理雌蚊幼鱼21 d，采用形态学和体外法定量测定其体长(BL)、体重(BW)、身体健康指数(CF)、肛门鳍第三段长度(FL)、截面数(FJ)、最宽部宽度(FW)以及ARα mRNA和vpg α mRNA的表达水平，并设置对照组和平行组。结果表明,提单和CF的女性青少年暴露于不同浓度的MT 21 d没有显著不同,只有BW鱼暴露在50和500 nmol / L高剂量组显著降低(P0.05)与对照组相比;第三鳍的FJ臀鳍显示显著增加,脂肪肝以及扩展和弗兰克-威廉姆斯也显示出显著增加宽度(P0.05);女性青少年暴露于不同浓度的MT在21d,ARα mRNA表达量呈剂量相关性升高(P0.05)。结果表明，MT的雄激素效应非常显著，导致雌性食蚊鱼在生长发育过程中出现形态上的雄性化;ARα mRNA表达水平可作为监测水产养殖水体雄激素污染物的理想生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

水产学报 Environmental Science-Management, Monitoring, Policy and Law

CiteScore

1.40

自引率

0.00%

发文量

5213

期刊介绍： "Fisheries of" mainly reflects the results of scientific research and development of the direction of aquaculture for domestic and foreign academic exchanges Fisheries Service. Mainly basic research published in Fisheries, aquaculture and proliferation of fishing waters environmental protection, preservation of aquatic products processing and utilization, fishing equipment, and other aspects of mechanical papers, research briefings and reviewed.