Immunogenic and protective effects of a DNA vaccine containing flagellin flaC gene against Vibrio alginolyticus in red snapper(Lutjanus sanguineus)

Q4 Environmental Science

水产学报 Pub Date : 2013-01-01 DOI:10.3724/SP.J.1231.2013.38236

Haiying Liang, Yongxin Cheng, J. Jian, Zao-he Wu

{"title":"Immunogenic and protective effects of a DNA vaccine containing flagellin flaC gene against Vibrio alginolyticus in red snapper(Lutjanus sanguineus)","authors":"Haiying Liang, Yongxin Cheng, J. Jian, Zao-he Wu","doi":"10.3724/SP.J.1231.2013.38236","DOIUrl":null,"url":null,"abstract":"In order to study the immunogenic and protective effects of DNA vaccine,plasmid DNA encoding flagellin flaC gene(designated as pcDNA-flaC)was used as a DNA vaccine to immunize red snapper(Lutjanus sanguineus).The distribution,expression and immunoprotection of the DNA vaccine were analyzed in tissues of the red snapper by PCR,RT-PCR and challenge test.PCR results indicated that pcDNA-flaC was distributed in liver,spleen,kidney,gill and injection site muscle at 7-28 days after vaccination.RT-PCR results indicated that the flaC gene was expressed in all above tissues of vaccinated fish at 7-28 days after vaccination.These results demonstrated that the DNA vaccine was distributed and flaC gene was expressed in various tissues of vaccinated fish.Red snapper immunized with DNA vaccine showed higher serum antibody levels at 7-28 days after vaccination,compared to fish vaccinated with the control eukaryotic expression vector pcDNA3.1 and PBS.In addition,fish immunized with DNA vaccine developed a protective response to live V.alginolyticus challenge 28 days post-inoculation,as demonstrated by increased survival of vaccinated fish over the control fish.This study indicates that pcDNA-flaC is an effective vaccine candidate against V.alginolyticus infection.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"1 1","pages":"125"},"PeriodicalIF":0.0000,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"水产学报","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.3724/SP.J.1231.2013.38236","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Environmental Science","Score":null,"Total":0}

引用次数: 0

Abstract

In order to study the immunogenic and protective effects of DNA vaccine,plasmid DNA encoding flagellin flaC gene(designated as pcDNA-flaC)was used as a DNA vaccine to immunize red snapper(Lutjanus sanguineus).The distribution,expression and immunoprotection of the DNA vaccine were analyzed in tissues of the red snapper by PCR,RT-PCR and challenge test.PCR results indicated that pcDNA-flaC was distributed in liver,spleen,kidney,gill and injection site muscle at 7-28 days after vaccination.RT-PCR results indicated that the flaC gene was expressed in all above tissues of vaccinated fish at 7-28 days after vaccination.These results demonstrated that the DNA vaccine was distributed and flaC gene was expressed in various tissues of vaccinated fish.Red snapper immunized with DNA vaccine showed higher serum antibody levels at 7-28 days after vaccination,compared to fish vaccinated with the control eukaryotic expression vector pcDNA3.1 and PBS.In addition,fish immunized with DNA vaccine developed a protective response to live V.alginolyticus challenge 28 days post-inoculation,as demonstrated by increased survival of vaccinated fish over the control fish.This study indicates that pcDNA-flaC is an effective vaccine candidate against V.alginolyticus infection.

查看原文本刊更多论文

含鞭毛蛋白flaC基因DNA疫苗对红鲷鱼溶藻弧菌的免疫原性及保护作用

为研究DNA疫苗的免疫原性和保护作用，采用编码鞭毛蛋白flaC基因的质粒DNA(简称pcDNA-flaC)作为DNA疫苗对血红鲷鱼进行免疫。采用PCR、RT-PCR和攻毒试验分析了DNA疫苗在红鲷鱼组织中的分布、表达和免疫保护作用。PCR结果显示，接种后7 ~ 28 d, pcDNA-flaC分布于肝脏、脾脏、肾脏、鳃和注射部位肌肉。RT-PCR结果显示，接种后7 ~ 28 d, flaC基因在接种鱼的上述组织中均有表达。结果表明，DNA疫苗在接种鱼的各个组织中均有分布和flaC基因的表达。与对照真核表达载体pcDNA3.1和PBS接种的红鲷鱼相比，接种DNA疫苗后7-28 d的血清抗体水平较高。此外，接种DNA疫苗的鱼在接种后28天对溶藻假弧菌的活攻击产生了保护性反应，这表明接种疫苗的鱼比对照鱼的存活率更高。本研究提示pcDNA-flaC是一种有效的抗溶藻弧菌感染的候选疫苗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

水产学报 Environmental Science-Management, Monitoring, Policy and Law

CiteScore

1.40

自引率

0.00%

发文量

5213

期刊介绍： "Fisheries of" mainly reflects the results of scientific research and development of the direction of aquaculture for domestic and foreign academic exchanges Fisheries Service. Mainly basic research published in Fisheries, aquaculture and proliferation of fishing waters environmental protection, preservation of aquatic products processing and utilization, fishing equipment, and other aspects of mechanical papers, research briefings and reviewed.