Proteomic Analysis Provides Insights into Arabidopsis thaliana Response Under Narrow-Wavelength LED of 595 nm light

Abstract

A growing body of evidence has highlighted that a wide range of plant processes including growth, photosynthesis, and stress response are regulated by 595 nm light. However, the molecular mechanisms underlying 595 nm-induced signals are not known. The aim of this work was to study the comparative proteomic changes in leaves of Arabidopsis thaliana Col-0 plants treated with narrow-wavelength 595 nm light or fluorescent light for 5 days. Harvested plant samples were analyzed using inline RP-SCX-RP liquid chromatography coupled with LTQ mass spectrometer, resulting in the identification of 1538 proteins. Linear regression modeling of proteins’ relative abundance revealed a total of 23 differentially abundant proteins (DAPs). Functional analysis of these DAPs demonstrated the role of several biological mechanisms in A. thaliana’s response to 595 nm light including stress response and metabolic processes. A network analysis of these DAPs revealed the importance of energy and redox regulation mechanisms. Further analyses determined potentially important roles for proteins associated with glycolysis, ATP synthase complex, cell wall modification, and thylakoid membrane that may modulate the plant’s adaptive response to 595 nm light. A significant enrichment of DAPs for PSII tolerance capacity, as well as associated Ca2+ and ROS signaling pathways were also identified. Collectively, this study provides an important insight into potential molecular pathways that sustain a plant’s response to 595 nm light.