Anti-Tn human monoclonal antibodies generated following active immunization with partially desialylated ovine submaxillary mucin.

Human antibodies and hybridomas Pub Date : 1994-01-01 DOI:10.3233/HAB-1994-51-204

K. O’Boyle, K. Wright

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引用次数: 6

Abstract

Partially desialylated ovine submaxillary mucin plus an immunological adjuvant has been used by us to induce a humoral immune response to Tn antigen and sialylated Tn in colon cancer patients at risk of recurrence. Peripheral blood lymphocytes were purified from one of these patients with a high IgM titer reactive with Tn antigen transformed with Epstein-Barr virus and subsequently fused with a human-mouse heteromyeloma cell line, HMMA2.11TG/O. Clones were screened and subcloned using an enzyme-linked immunoassay and four stable IgM-secreting clones were tested for reactivity with a variety of natural and synthetic antigens, paraffin-embedded colon carcinomas and normal colonic mucosa to determine their specificity. Four human IgM monoclonal antibodies reactive predominantly with Tn antigen were produced and shown to react with a mucinous human colon carcinoma cell line, LS-174T, and with paraffin-embedded human colon carcinomas. We conclude that modified ovine submaxillary mucin is an effective vaccine for generating a humoral immune response directed to Tn antigen present on human colon cancer.

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部分脱氮羊颌下黏液蛋白主动免疫后产生的抗tn人单克隆抗体。

我们已经使用部分去盐化的羊颌下黏液蛋白加上免疫佐剂，在有复发风险的结肠癌患者中诱导对Tn抗原和唾液化Tn的体液免疫反应。其中一名患者的外周血淋巴细胞具有高IgM滴度，与Epstein-Barr病毒转化的Tn抗原反应，随后与人-小鼠异骨髓瘤细胞系HMMA2.11TG/O融合。利用酶联免疫分析法对克隆进行筛选和亚克隆，并检测4个稳定的igm分泌克隆对多种天然和合成抗原、石蜡包埋结肠癌和正常结肠粘膜的反应性，以确定它们的特异性。制备了四种主要与Tn抗原反应的人IgM单克隆抗体，并显示它们与黏液性人结肠癌细胞系LS-174T和石蜡包埋的人结肠癌发生反应。我们得出结论，改良的羊颌下黏液蛋白是一种有效的疫苗，可产生针对人结肠癌上存在的Tn抗原的体液免疫反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Human antibodies and hybridomas

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