Infrared spectroscopy as a new tool for studying single living cells: Is there a niche?

IF 0.3 Q4 SPECTROSCOPY

Biomedical Spectroscopy and Imaging Pub Date : 2017-01-01 DOI:10.3233/BSI-170171

S. Sabbatini, C. Conti, G. Orilisi, E. Giorgini

引用次数: 39

Abstract

FTIR spectroscopy is an analytical technique widely applied for studying the vibrational fingerprint of organic compounds. In recent years, it has been applied to many biomedical fields because of its potential to detect the composition and molecular structure of various biological materials without the need of probe molecules. The coupling of IR spectrometers with visible microscopes has led to perform the imaging analysis of non-homogeneous samples, such as tissues and cells, in which the biochemical and spatial information are close related. In this review, we report the most significant applications of FTIR to the study of cells in different conditions (fixed, dried and living) with the aim to monitor their biochemical modifications, either induced or naturally occurring.

查看原文本刊更多论文

红外光谱作为研究单个活细胞的新工具:是否有一席之地?

FTIR光谱是一种广泛应用于有机化合物振动指纹图谱研究的分析技术。近年来，由于其无需探针分子即可检测各种生物材料的组成和分子结构的潜力，已被应用于许多生物医学领域。红外光谱仪与可见显微镜的耦合使得对非均匀样品(如组织和细胞)进行成像分析，其中生化和空间信息密切相关。在这篇综述中，我们报告了FTIR在不同条件下(固定、干燥和活)细胞研究中的最重要应用，目的是监测它们的生化修饰，无论是诱导的还是自然发生的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomedical Spectroscopy and Imaging SPECTROSCOPY-

自引率

0.00%

发文量

期刊介绍： Biomedical Spectroscopy and Imaging (BSI) is a multidisciplinary journal devoted to the timely publication of basic and applied research that uses spectroscopic and imaging techniques in different areas of life science including biology, biochemistry, biotechnology, bionanotechnology, environmental science, food science, pharmaceutical science, physiology and medicine. Scientists are encouraged to submit their work for publication in the form of original articles, brief communications, rapid communications, reviews and mini-reviews. Techniques covered include, but are not limited, to the following: • Vibrational Spectroscopy (Infrared, Raman, Teraherz) • Circular Dichroism Spectroscopy • Magnetic Resonance Spectroscopy (NMR, ESR) • UV-vis Spectroscopy • Mössbauer Spectroscopy • X-ray Spectroscopy (Absorption, Emission, Photoelectron, Fluorescence) • Neutron Spectroscopy • Mass Spectroscopy • Fluorescence Spectroscopy • X-ray and Neutron Scattering • Differential Scanning Calorimetry • Atomic Force Microscopy • Surface Plasmon Resonance • Magnetic Resonance Imaging • X-ray Imaging • Electron Imaging • Neutron Imaging • Raman Imaging • Infrared Imaging • Terahertz Imaging • Fluorescence Imaging • Near-infrared spectroscopy.