The effect of amifostine on Survivin expression in HL-60 cells in vitro

山西医科大学学报 Pub Date : 2007-01-01 DOI:10.3760/CMA.J.ISSN.1009-9921.2008.04.005

Liang-ming Ma, Yong-an Zhou, Lei Zhu

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Abstract

Objective To explore the mechanisms of apoptosis induced by arsenic trioxide and amifostine in human acute promyelocytic leukemia cell lines HL-60 in vitro.Methods HL-60 cells were treated with different concentrations of arsenic trioxide alone and combined with amifostine.The inhibitory ratio of the ceils were measured by MTT assay.and the expression of Survivin Was detected by semiquantitate RT-PCR.Results Proliferation of HL-60 cells exposed to arsenic trioxide dwpped down with increasing dose of the dmg and this effect Was significantly hisher when arsenic trioxide Was used in combination with amifostine.Furthermore.there was a more significant decrease in Survivin expression in HL-60 cells treated with arsenic trioxide in combination with amifostine as compared to the cells treated only with arsenic trioxide.Conclusion Arsenic trioxide induced HL-60 cells to undergo apoptosis by downregulating the expression of Survivin. Amifostine enhanced the sensitivity of HL-60 cells to arsenic trioxide by downregulating the expression of Survivin,thus promoting apoptosis effect. Key words: Arsenical; Amifostine; Cytoprotection; HL-60 cell; Survivin gene

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氨磷汀对HL-60细胞Survivin表达的影响

目的探讨三氧化二砷和氨磷汀诱导人急性早幼粒细胞白血病HL-60细胞体外凋亡的机制。方法分别用不同浓度的三氧化二砷和氨磷汀对HL-60细胞进行处理。MTT法测定细胞的抑制率。半定量RT-PCR检测Survivin的表达。结果三氧化二砷对HL-60细胞的增殖作用随着剂量的增加而下降，且三氧化二砷联合氨磷汀对细胞增殖的影响更明显，且三氧化二砷联合氨磷汀对HL-60细胞Survivin表达的抑制作用比单用三氧化二砷的作用更显著。结论三氧化二砷通过下调Survivin的表达诱导HL-60细胞凋亡。氨磷汀通过下调Survivin的表达增强HL-60细胞对三氧化二砷的敏感性，从而促进细胞凋亡作用。关键词:砷;Amifostine;Cytoprotection;HL-60细胞;生存素基因

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来源期刊

山西医科大学学报

自引率

0.00%

发文量

11408

期刊介绍： Journal of Shanxi Medical University is a comprehensive medical academic journal sponsored by Shanxi Medical University and supervised by the Shanxi Provincial Department of Education. It mainly publishes scientific research results and work experience in basic medicine, preventive medicine, and clinical medicine. The main columns of the journal include tumor research, heart and cardiovascular disease, liver and liver disease, basic medicine, pharmacy, clinical medicine, reviews and opinions, methods and techniques, case reports, etc.