Composition of Bacterial Community in Musca domestica Midgut

Q4 Agricultural and Biological Sciences

应用与环境生物学报 Pub Date : 2013-12-13 DOI:10.3724/SP.J.1145.2013.00800

Liu Yang, Zhonghua Wei, Fei Zhongan, Zhao Feng, Qiao Dai-rong, C. Yi

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引用次数: 0

Abstract

Housefly(Musca domestica) is an important mechanical and biological vector harboring and transmitting human and animal pathogens. To study the composition of bacterial community in housefly midgut and assess the role of housefly in the epidemiology of diseases, 16S rRNA gene clone library of bacteria in housefly midgut was constructed. The library compromised of 88 sequences, which were grouped into 22 OTUs, including Proteobacteria(12 OTUs), Firmicutes(9 OTUs), and Bacteroidetes(1 OTU). Twelve OTUs of Proteobacteria belonged to Betaproteobacteria(1 OTU), Epsinoproteobacteria(1 OTU)and Gammaproteobacteria(10 OTUs) respectively. No Alphaproteobacteria or Deltaproteobacteria was found. The 9 Firmicutes included Lactobacillales(6 OTUs), Clostridia(1 OTU) and Bacillales(2 OTUs). In the library, Gammaproteobacteria was the dominant phylotype, which compromised 71 sequences(80.7%). Some species in Enterobacter, Klebsiella, Providencia, Proteus,Aeromonas, and Staphylococcus in the library were pathogens or opportunistic pathogens to human and animal. These results help to understand the significant roles of housefly in disease transmitting.

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家蝇中肠细菌群落组成

家蝇(Musca domestica)是一种重要的机械和生物媒介，是人类和动物病原体的窝藏和传播媒介。为研究家蝇中肠细菌群落组成，评价家蝇在疾病流行病学中的作用，构建家蝇中肠细菌16S rRNA基因克隆文库。该文库包含88个序列，分为22个OTU，包括变形菌门(12个OTU)、厚壁菌门(9个OTU)和拟杆菌门(1个OTU)。Proteobacteria中12个OTU分别属于Betaproteobacteria(1个OTU)、epsinproteobacteria(1个OTU)和Gammaproteobacteria(10个OTU)。未发现α变形菌和δ变形菌。9门厚壁菌门包括乳酸杆菌门(6 OTU)、梭状芽胞杆菌门(1 OTU)和芽胞杆菌门(2 OTU)。在文库中，Gammaproteobacteria为优势种型，有71个序列(80.7%)被破坏。文库中肠杆菌、克雷伯氏菌、普罗维登氏菌、变形杆菌、气单胞菌和葡萄球菌部分为人畜致病菌或条件致病菌。这些结果有助于了解家蝇在疾病传播中的重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

应用与环境生物学报 Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.20

自引率

0.00%

发文量

4458

期刊介绍： The “Chinese Journal of Applied and Environmental Biology” is a national scientific and technological journal published both at home and abroad. It was started in 1995 and became bimonthly in 1999, and now it is a core journal for applied and environmental biology in China. It aims to introduce and exchange the new achievements, new technologies, new methods and new progresses made from the research fields of biology and its related disciplines both in China and abroad; enhance scientific and technological research serving for the development and construction of national economy, and improvement of people’s living standards.