Screening and Identification of a Polyhydroxyalkanoate Producing Strain Rhizobium sp. H2-5

Abstract

Polyhydroxyalkanoate(PHA) is a family of carbon and energy storage materials synthesized by many bacteria.PHA,as a kind of degradable material,can be used in many areas,such as bioplastics,medical materials etc.In this study,several soil or water samples were collected from environment,and a polyhydroxyalkanoate producing strain was isolated from soil by enrichment culturing,Nile blue fluorescence staining,Sudan black staining,and Fourier transform infrared spectra elemental analysis.This strain was identified and named as Rhizobium sp.H2-5 according to its morphological features,physiological and biochemical tests as well as 16S rDNA gene sequence analysis.