Change of gydrolase and oxide reductase fermental complex activity and indicators of fertility to sod-podsolic soil under action of bentonite clay

A. Kozlov, A. Kulikova, O. Selitskaya, I. Uromova
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Abstract

In aspect of modern direction of potential impact assessment of alternative fertilizer materials on properties of soils variability of activity of the main gydrolase and oxide reductase enzymes in sod-podsolic sandy loamy soil (by WRB Retisols) and indicators of its effective fertility under influence of meliorative doses of bentonite clay is determined. Microfield research was conducted during 2015–2017 in conditions of the Nizhny Novgorod Region. Breed was brought once (2014) in an arable layer of earth in doses at rate of 3, 6 and 12 t/hectare. In years of carrying out research grew up winter wheat (Moskovskaya 39), barley (Veles) and peas of sowing campaign (Chishminsky 95). When determining enzymatic activity of soil and maintenance of batteries of plants in it used classical biochemical and agrochemical methods used in soil science. Under influence of bentonite clay high doses in soil observed considerable activization of enzymatic activity (protease (to 90%), cellulase (almost twice), phosphatase (up to 140%), phenol oxidase (up to 30%) and catalase (up to 70%)). As result, improvement of its agrochemical indicators is revealed, including increase in content of mobile compounds of phosphorus (on 21–31 mg/kg), exchange forms of potassium (on 32–41 mg/kg), available silicates (by 2–8 times in α-form and at 1,5–3,0 time in β-form), strengthening of nitrifying ability (on 2,6–4,1 mg/kg) and also relative persistence of maintenance of humic substances at level of control is established. Received results can be basis for introduction of bentonite in production field experiments and in subsequent for development of technology of bentonite clays use in agriculture of the Non-chernozem zone as meliorative substance, optimizing enzymatic activity of soils.
膨润土作用下豆灰土水解酶和氧化还原酶发酵复合体活性及肥力指标的变化
从评价替代肥料材料对土壤性质潜在影响的现代方向出发,确定了膨润土改良剂量对软土-灰土砂质壤土中主要水解酶和氧化还原酶活性的变异性及其有效肥力指标。微场研究于2015-2017年在下诺夫哥罗德地区进行。该品种曾于2014年在可耕土层中以3、6和12吨/公顷的剂量施用。在多年的研究中,培育出冬小麦(Moskovskaya 39)、大麦(Veles)和豌豆(Chishminsky 95)。在测定土壤酶活性和维持土壤中植物电池时,使用了土壤学中常用的经典生化和农化方法。在高剂量的膨润土粘土的影响下,观察到土壤中酶活性(蛋白酶(高达90%)、纤维素酶(几乎翻倍)、磷酸酶(高达140%)、酚氧化酶(高达30%)和过氧化氢酶(高达70%))的显著活化。结果表明,其农化指标得到改善,包括磷的流动化合物含量(21 ~ 31 mg/kg),钾的交换形式(32 ~ 41 mg/kg),有效硅酸盐(α-形式增加2 ~ 8倍,β-形式增加1、5 ~ 3、0倍),硝化能力增强(2、6 ~ 4、1 mg/kg),腐殖质维持在控制水平的相对持久性。所得结果可作为将膨润土引入生产现场试验的基础,并为今后开发膨润土在非黑钙土区农业中作为改良物质、优化土壤酶活性的技术奠定基础。
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