Toxic impacts of a mixture of three pesticides on the reproduction and oxidative stress in male rats

IF 1.8 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Animal Behaviour and Biometeorology Pub Date : 2021-01-01 DOI:10.31893/jabb.22004

Narimene Bouabdallah, L. Mallem, C. Abdennour, A. Chouabbia, Mohamed Tektak

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引用次数: 7

Abstract

The present study investigated the toxic effect of a mixture of three pesticides (cypermethrin, mancozeb, and metalaxyl) on reproduction and oxidative stress parameters in male Wistar rats. Animals were treated at doses 1/60, 1/30, and 1/10 LD50 of each pesticide daily in the diet for 08 weeks. At the end of the treatment period, animals were sacrificed by decapitation. The results indicate a decrease in the absolute weight of testes and epididymis, the serum of testosterone hormone, and cholesterol levels. These parameters were significant reduced in males exposed to the mixed pesticides. A reduction in sperm concentration, motility, and viability also was observed. Besides, the ingestion of mixed pesticides at all three concentrations caused a significant decrease in GSH, GPx levels and an increase in MDA levels compared to the control group. This was accompanied by histopathological changes in testis and epididymis of rats such as seminiferous tubules degeneration, decreasing number of spermatogenic cells, edema, expansion of interstitial spaces, cell necrosis, and reducing the diameter of the epididymal tube compared to the control group. Thus, we strongly suggest that the mixture of pesticides causes damages to the male reproductive system.

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三种农药混合物对雄性大鼠生殖和氧化应激的毒性影响

本研究研究了氯氰菊酯、代锰锌和甲螨灵三种农药混合施用对雄性Wistar大鼠生殖和氧化应激参数的毒性作用。各组动物分别在日粮中添加1/60、1/30和1/10 LD50的农药，试验期为08周。在治疗期结束时，动物被斩首牺牲。结果表明，睾丸和附睾的绝对重量、血清睾酮激素和胆固醇水平均有所下降。这些参数在接触混合农药的雄性中显著降低。还观察到精子浓度、活力和生存能力的降低。此外，与对照组相比，摄入三种浓度的混合农药导致GSH、GPx水平显著降低，MDA水平显著升高。与对照组相比，大鼠睾丸和附睾出现精管变性、生精细胞数量减少、水肿、间质肿大、细胞坏死、附睾管直径减小等组织病理学改变。因此，我们强烈建议农药混合物对男性生殖系统造成损害。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Animal Behaviour and Biometeorology Environmental Science-Ecology

CiteScore

4.00

自引率

9.50%

发文量

期刊介绍： The Journal of Animal Behaviour and Biometeorology (ISSN 2318-1265) is the official journal of the Center for Applied Animal Biometeorology (Brazil) currently published by Malque Publishing. Our journal is published quarterly, where the published articles are inserted into areas of animal behaviour, animal biometeorology, animal welfare, and ambience: farm animals (mammals, birds, fish, and bees), wildlife (mammals, birds, fish, reptiles, and amphibians), pets, animals in zoos and invertebrate animals. The publication is exclusively digital and articles are freely available to the international community. Manuscript submission implies that the data are unpublished and have not been submitted for publication in other journals. JABB publishes original articles in the form of Original Articles, Short Communications, and Reviews. Original Articles arising from research work should be well grounded in theory and execution should follow the scientific methodology and justification for its objectives; Short Communications should provide sufficient results for a publication in accordance with the Research Article; Reviews should involve the relevant scientific literature on the subject. JABB publishes articles in English only. All articles should be written strictly adopting all the rules of spelling and grammar.