Immobilization of Papain on Siliceous Mesocellular Foam

Q4 Materials Science

Cailiao Daobao/Materials Review Pub Date : 2009-01-01 DOI:10.3724/sp.j.1077.2009.00702

Nie Zuoren

引用次数: 1

Abstract

Siliceous mesocellular foam(MCF)is employed as carriers in the immobilization of papain,and the properties and the stabilities of the immobilized enzyme are investigated in detail.The results show that the amount of papain immobilized on MCF reaches 334mg/g MCF and the activity of immobilized papain is 95.3% of that of the free papain.The Km of immobilized and free papain are 6.99×10-3mol/L and 6.71×10-3mol/L,respectively.The thermal and storage stabilities of immobilized enzyme are improved due to the microenvironment and the shield of the mesopores.MCF can be considered as excellent supports for papain immobilization.

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硅质中孔泡沫固定化木瓜蛋白酶

以硅质介孔泡沫(MCF)为载体，对固定化酶的性质和稳定性进行了研究。结果表明，在MCF上固定化木瓜蛋白酶的量达到334mg/g MCF，固定化木瓜蛋白酶的活性为游离木瓜蛋白酶的95.3%。固定化和游离木瓜蛋白酶的Km分别为6.99×10-3mol/L和6.71×10-3mol/L。微环境和介孔的屏蔽作用提高了固定化酶的热稳定性和贮存稳定性。MCF可以被认为是木瓜蛋白酶固定的良好支撑。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cailiao Daobao/Materials Review Materials Science-Materials Science (all)

CiteScore

1.20

自引率

0.00%

发文量

1802

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