Evaluation of Serum-Free, Xeno-Free Cryopreservation Solutions for Human Adipose-Derived Mesenchymal Stem Cells.

Cell medicine Pub Date : 2017-01-01 DOI:10.3727/215517916X693122

Chika Miyagi-Shiohira, N. Kobayashi, I. Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, H. Noguchi

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引用次数: 18

Abstract

Adipose-derived mesenchymal stem cells (ASCs) have the potential to differentiate into cells of mesodermal origin, such as osteoblasts, adipocytes, myocytes, and chondrocytes, and cryopreservation is currently performed as a routine method for preserving ASCs to safely acquire large numbers of cells. For clinical application of ASCs, serum-free, xeno-free cryopreservation solutions should be used. This study determined the viability and adipo-osteogenic potential of cryopreserved ASCs using four cryopreservation solutions: 10% DMSO, Cell Banker 2 (serum free), Stem Cell Banker (=Cell Banker 3: serum free, xeno free), and TC protector (serum free, xeno free). The viability of the cryopreserved ASCs was over 80% with all cryopreservation solutions. No difference in the adipo-osteogenic potential was found between the cells that did or did not undergo cryopreservation in these cryopreservation solutions. These data suggest that Cell Banker 3 and TC protector are comparable with 10% DMSO and Cell Banker 2 for ASCs, and cryopreserved as well as noncryopreserved ASCs could be applied for regenerative medicine.

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人脂肪源性间充质干细胞无血清、无xeno冷冻保存液的评价。

脂肪来源的间充质干细胞(ASCs)具有分化为中胚层细胞的潜力，如成骨细胞、脂肪细胞、肌细胞和软骨细胞，目前冷冻保存是保存ASCs以安全获得大量细胞的常规方法。对于临床应用的ASCs，应使用无血清、无异种冷冻保存液。本研究采用四种冷冻保存溶液:10% DMSO、Cell Banker 2(无血清)、Stem Cell Banker (=Cell Banker 3:无血清、无xeno)和TC protector(无血清、无xeno)，测定冷冻保存的ASCs的活力和脂肪成骨潜能。冷冻保存的ASCs的存活率均在80%以上。在这些冷冻保存溶液中进行冷冻保存或未进行冷冻保存的细胞之间，脂肪成骨潜能没有差异。这些数据表明，对于ASCs, Cell Banker 3和TC protector与10% DMSO和Cell Banker 2相当，冷冻保存和非冷冻保存的ASCs都可以应用于再生医学。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cell medicine MEDICINE, RESEARCH & EXPERIMENTAL-

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