Behavior of Human Articular Chondrocytes During In Vivo Culture in Closed, Permeable Chambers.

I. Martinez-Zubiaurre, Tuija Annala, M. Polacek
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引用次数: 3

Abstract

The exact contribution of transplanted chondrocytes for cartilage tissue repair prior expansion in monolayer culures remains undetermined. At our laboratory, we have created a new permeable chamber to study the chondrogenesis of dedifferentiated cells implanted ectopically in a closed and controlled environment. The behavior of chondrocytes has been studied in settings frequently used in clinical approaches during transplantation, namely injection of autologous chondrocyte cells in suspension (ACI), cells soaked in collagen membranes (MACI), and cells applied in a polymer gel (fibrin). As controls, we have tested the redifferentiation of chondrocytes in cell aggregates, and we have checked the proper functionality of chambers both in vitro and in vivo. After retrieval, firmed tissue-like shapes were recovered only from chambers containing cells seeded in membranes. Histomorphological, immunohistochemical, and ultrastructural analyses revealed synthesis of fibrous-like tissue, characterized by low-density collagen fibers, low collagen type II, abundant collagen type I, and low amounts of proteoglycans. Additionally, neither the collagen membranes nor the fibrin gel was reabsorbed by cells. In summary, our results show that the newly developed permeable chambers function correctly, allowing proper cell feeding and preventing cell leakage or host cell invasion. Additionally, our results suggest that, under these circumstances, chondrocytes are not able to orchestrate formation of hyaline cartilage and have little capacity to degrade artificial membranes or carrier gels such as fibrin. These are interesting observations that should be considered for understanding what role the transplanted chondrocytes play during restoration of articular cartilage after implantation.
人关节软骨细胞在封闭、可渗透腔体内培养过程中的行为。
在单层培养中,移植软骨细胞对软骨组织修复的确切贡献尚未确定。在我们的实验室,我们创造了一个新的可渗透室,用于研究在封闭和控制的环境中异位植入的去分化细胞的软骨形成。软骨细胞的行为已经在移植过程中经常使用的临床方法中进行了研究,即注射悬浮中的自体软骨细胞(ACI),浸泡在胶原膜中的细胞(MACI),以及应用于聚合物凝胶(纤维蛋白)中的细胞。作为对照,我们在细胞聚集体中测试了软骨细胞的再分化,并在体外和体内检查了腔室的正常功能。回收后,仅从含有膜内细胞的腔室中恢复了固定的组织样形状。组织形态学、免疫组织化学和超微结构分析显示合成了纤维样组织,其特征是低密度胶原纤维、低II型胶原、丰富的I型胶原和低量的蛋白多糖。此外,胶原膜和纤维蛋白凝胶都没有被细胞重吸收。总之,我们的研究结果表明,新开发的渗透腔功能正确,允许适当的细胞摄食,防止细胞渗漏或宿主细胞入侵。此外,我们的研究结果表明,在这种情况下,软骨细胞不能协调透明软骨的形成,并且几乎没有能力降解人工膜或纤维蛋白等载体凝胶。这些有趣的观察结果对于理解移植的软骨细胞在植入后关节软骨的修复中所起的作用是值得考虑的。
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来源期刊
Cell medicine
Cell medicine MEDICINE, RESEARCH & EXPERIMENTAL-
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